高脂饲料中添加绿原酸对草鱼生长性能和脂质代谢的影响  

Effects of Chlorogenic Acid Supplementation in High-Fat Diets on Growth Performance and Lipid Metabolism of Grass Carp(Ctenopharyngodon idellus)

作　　者：杨天俊 陈彦良[1] 刘文舒 郭小泽[1] 唐艳强[1] 刘玉廷 黎徳兵 李思明[1] YANG Tianjun1,2, CHEN Yanliang1, LIU Wenshu1 ,GUO Xiaoze1 ,TANG Yanqiang1 ,LIU Yuting1,2, LI Debing2, LI Siming1.(1. Jiangxi Academy of Agricultural Science, Nanchang 330200, China; 2. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Chin)

机构地区：[1]江西省农业科学院,南昌330200 [2]四川农业大学动物科技学院,成都611130

出　　处：《动物营养学报》2018年第8期3219-3228,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金地区科学基金项目(31660743);江西省特种水产产业技术体系(JXARS-10);江西现代农业科研协同创新专项(JXXTCX2015004-009;JXXTC201602-03);江西省青年科学基金项目(20171BAB214015)

摘　　要：本试验旨在探讨高脂饲料中添加绿原酸(CGA)对草鱼生长性能和脂质代谢的影响。选择健康、初始体重为(7.53±0.30)g的草鱼360尾,随机分为4组,C(对照)、200CGA、400CGA、600CGA组分别饲喂CGA添加水平为0、200、400、600 mg/kg的高脂饲料(粗脂肪含量约为9.00%),每组3重复,每重复30尾,饲喂11周。结果表明,与C组相比:1)400CGA组鱼终末体重、增重率、蛋白质效率均显著提高(P<0.05),其中终末体重增加15.90%,增重率提高22.96%,蛋白质效率提升17.75%,同时饲料系数显著降低(P<0.05),降低14.86%;2)200CGA、400CGA组血清总胆固醇(T-CHO)、低密度脂蛋白胆固醇(LDL-C)含量显著降低(P<0.05),试验组血清高密度脂蛋白胆固醇(HDL-C)含量显著提高(P<0.05);3)试验组肝胰脏甘油三酯(TG)、T-CHO、LDL-C含量显著降低(P<0.05),肝胰脏肝脂酶(HL)、总脂酶(TL)活力显著提高(P<0.05),400CGA、600CGA组肝胰脏HDL-C含量显著提高(P<0.05),400CGA组肝胰脏脂蛋白脂酶(LPL)活力显著提高(P<0.05);4)透射电镜切片结果显示,400CGA、600CGA组肝胰脏细胞内脂滴体积小、数量少、分布较稀疏。结果提示,高脂饲料中CGA添加水平为400 mg/kg时,可促进草鱼机体脂质代谢,改善生长性能。This study aimed to investigate the effects of chlorogenic acid （CGA） supplementation in high-fat diets on growth performance, lipid metabolism of grass carp（ Ctenopharyngodon idellus ）. A total of 360 healthy and grass carp with an initial body weight （7.53±0.30） g were randomly divided into 4 groups with 3 replicates per group and 30 craps per replicate. Craps in C （control）, 200CGA, 400CGA and 600CGA groups were fed high-fat diet （crude lipid was about 9.00%） supplemented with 0, 200, 400 and 600 mg/kg CGA for 11 weeks, respectively. The results showed that compared with C group： 1） 400CGA group had significantly higher final body weight （FBW）, weight gain rate （WGR） and protein efficiency ratio （PER） of diet （ P 〈0.05）, FBW increased by 15.90% , WGR increased by 22.96%m and PER increased by 17.75%, 400CGA group had significantly lower feed coefficient （ P 〈0.05）, which was decreased by 14.86%. 2） 200CGA and 400CGA groups had significantly lower serum contents of total cholesterol （T-CHO） and low density lipoprotein cholesterol （LDL-C） （ P 〈0.05）, experimental groups had significantly higher serum content of high density lipoprotein cholesterol （HDL-C） （ P 〈0.05）. 3） Experimental groups had significantly lower contents of triglyceride （TG）, T-CHO and LDL-C in hepatopancreas （ P 〈0.05）, and significantly higher activities of lipase（HL） and total lipase（TL） in hepatopancreas （ P 〈0.05）; 400CGA and 600CGA groups had significantly higher HDL-C content in hepatopancreas （ P 〈0.05）; 400CGA group had significantly higher lipoprotein lipase （LPL） activity in hepatopancreas （ P 〈0.05）. 4） Hepatopancreas sections under transmission electron microscope showed that lipid droplets were smaller, littleness and scattered in 400CGA and 600CGA groups. As a result, the supplementation of 400 mg/kg CGA in high-fat diets can promote lipid metabolism and growth performance of grass carp.

关 键 词：草鱼 绿原酸 高脂饲料 生长性能 脂质代谢 

分 类 号：S963[农业科学—水产养殖]