2株兔出血症病毒全基因测定与遗传进化分析  

Full genetic sequence determination and genetic evolution analysis of two rabbit hemorrhagic disease viruses

作　　者：蒙正群 周丽军[1] 罗怡琳[1] 冷依伊 张鹏飞[1] 王印[1,2] 姚学萍[1] 耿毅[1] 杨泽晓[1] MENG Zheng-qun;ZHOU Li-jun;LUO Yi-lin;LENG Yi-yi;ZHANG Peng-fei;WANG Yin;YAO Xue-ping;GENG Yi;YANG Ze-xiao(College of Veterinary Medicine,Sichuan Agricultural University,Wenjiang 611130,China;Key Laboratory of Animal,Disease and Human Health of Sichuan Province,Wenjiang 611130,China)

机构地区：[1]四川农业大学动物医学院,四川温江611130 [2]动物疫病与人类健康四川省重点实验室,四川温江611130

出　　处：《江苏农业学报》2018年第4期854-861,共8页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31402222);"十二五"国家科技支撑计划项目(2013BAD12B04);四川省科技支撑计划项目(2016NZ0002)

摘　　要：对2株兔出血症病毒(RHDV)SCH04、Sch07进行全基因组序列测定,并进行同源性及遗传进化分析。按照病毒核酸组成,将病毒基因分7段进行RT-PCR扩增,将分段扩增产物分别克隆到p MD-19T载体进行测序,用DNAStar进行拼接,得到全基因组序列;参照Gen Bank上登陆的31株RHDV毒株全基因核苷酸序列、VP60基因核苷酸序列以及ORF2编码基因核苷酸序列对2株病毒进行同源性和遗传进化分析。结果显示,SCH04基因组全长7 439 bp,Sch07基因组全长7 438 bp,2株病毒全基因的核苷酸序列同源性为99.8%,与31株参考毒株全基因的核苷酸序列同源性为78.3%~97.0%;2株病毒的VP60基因核苷酸序列同源性为99.7%,ORF2编码基因核苷酸序列同源性为99.2%。进化树显示2株病毒同属于抗原变异株RHDVa(GI.1a)基因群,且亲缘关系最近。VP60基因核苷酸序列与ORF2编码基因核苷酸序列均可以作为RHDV遗传进化分析。The complete genomes of two rabbit hemorrhagic disease viruses（ RHDV） SCH04,Sch07 were determinated,and the homologies and genetic evolution were analyzed in the study. The virus＇ s gene sequences were divided into seven sections for RT-PCR amplification according to the viral nucleic acid,and then the amplification products were cloned into p MD-19 T vector for sequencing,and complete genomes were spliced by DNAStar software. The homologies and genetic evolution were analyzed by comparing with 31 strains viral complete genomes,VP60 gene sequences,and ORF2 encoding gene sequences in Gen Bank. The results showed that fragment length of two strains complete genomes SCH04 and Sch07 were 7 439 bp and 7 438 bp,respectively. The homology of nucleotide sequence between strains complete genomes was 99. 8%,and the homologies of two strains with other 31 reference strains complete genomes were 78.3%-97.0%. The homology of VP60 gene sequences between two strains was 99. 7% and the homology of ORF2 encoding gene sequences between two strains was 99. 2%. Phylogenetic trees results showed the two strains belonged to the antigenic variation RHDVa（ GI.1 a） group,and genetic relationship was most recently. Both the VP60 gene sequences and ORF2 encoding gene sequences can be used for analyzing evolutionary relationship of RHDV.

关 键 词：兔出血症病毒 全基因 序列 遗传进化 

分 类 号：S858.291.265[农业科学—临床兽医学]