抗雄性激素和孕酮对6岁龄梅花鹿二茬茸生长发育的影响  

Effect of anti-androgen and progesterone on development of regrowth antlers in 6 year old sika deer

作　　者：赵海平[1] 崔学哲[1] 张伟[1] 陈广信 杨万云[1] 王权威 李春义[1,2] ZHAO Hai-ping;CUI Xue-zhe;ZHANG Wei;CHEN Guang-xin;YANG Wan-yun;WANG Quan-wei;LI Chun-yi(Institute of Special Animal and Plant Sciences of CAAS,State Key Laboratory for Molecular Biology of Special Economic Animals,Deer Antler Engineering Research Center,Changchun 130112,China;Changchun Sci-Tech University,Changchun 130600,China;College of Veterinary Medicine of Jilin University,Changchun 130000,China)

机构地区：[1]中国农业科学院特产研究所特种经济动物分子生物学国家重点实验室吉林省鹿茸工程研究中心,吉林长春130112 [2]长春科技学院,吉林长春130600 [3]吉林大学动物医学学院,吉林长春130000

出　　处：《中国中药杂志》2018年第13期2713-2719,共7页China Journal of Chinese Materia Medica

基　　金：吉林省重点科技攻关项目(20150204071NY)

摘　　要：为研究抗雄性激素药物和醋酸美伦孕酮对6岁龄梅花鹿二茬茸生长发育的影响,选择体质量和头茬茸产量相近的6岁龄健康梅花鹿20只,采用单因子试验设计,随机均分成5组。基础粮饲相同,对照组不做特殊处理,其余4组分别注射抗雄性激素氟他胺（flutamide）、康士得（bicalutamide）、醋酸环丙孕酮（progesterone acetate）和醋酸美伦孕酮（melengestrol acetate）。试验期60 d,共采集血液4次,测定梅花鹿血浆中雄性激素睾酮的含量,对比不同组变化规律;观察二茬茸生长发育状态;实验结束,梅花鹿二茬茸锯茸称重,计算二茬茸比重。结果发现,试验组二茬茸质量显著高于对照组,氟他胺、康士得、醋酸环丙孕酮、醋酸美伦孕酮与对照组相比分别增重100.50%,64.46%,87.16%,117.46%。试验早期（35 d）各组梅花鹿血液睾酮含量差异不显著;试验末期各组梅花鹿睾酮含量均极显著高于前一阶段（P〈0.01）;其中注射氟他胺组极显著低于其他组（P〈0.01）,康士得组和醋酸美伦孕酮组极显著高于其他组（P〈0.01）。结果表明对梅花鹿注射抗雄性激素和孕酮均促进了二茬茸的发育,增加了二茬茸的质量,其中以醋酸美伦孕酮组最为显著。从鹿茸的形态观察发现,抗雄性激素和孕酮处理延缓鹿茸骨化,从而延长了二茬茸的生长期,但是均没有影响血浆睾酮水平。To investigate the effects of anti-androgen drugs and melengestrol acetate（MGA） on development of regrowth antlers in6 year old sika deer,twenty healthysika deerwith similar body weight and antler weightwere randomly divided into five groups by using single factor test design： flutamide（n = 4）,bicalutamide（n = 4）,progesterone acetate（CPA,n = 4）,melengestrol acetate（MGA,n = 4）,control（n = 4）. All deer were fed with same diets and were housed outside together in an opened fence of 15 m × 30 m with free access to water and feed. Treatment groups were injected subcutaneously sustained-release agents of the four drugs respectively when two-branched antlers were harvested. The control group had no special treatment. In the experiment period of 60 d,blood sampleswere collected for 4 times for each deer. The concentration of testosterone in plasma was tested and analyzed to compare the changes between different groups. Development of regrowth antlers was observed. At the end of the experiment,regrowth antlers were weighted and analyzed. The resultsshowed that the weights of regrowth antlers in treatment groups were significantly greater than those from control group and the weight gain（as compared with the control group） was 100. 50%,64. 46%,87. 16% and 117. 46% respectively in flutamide group,bicalutamide group,progesterone acetate group and melengestrol acetate group. For plasma testosterone concentration,it was not significantly different in the early stage（in the first 35 d）,but at the end of the experimen,it was significantly higher than that of earlier stage（P 0. 01） in various groups. Testosterone concentration of flutamide treated group was significantly lower than that of the other groups（P 0. 01）,while the level inbicalutamide and MGA treated groups was significantly higher than that in other groups（P 0. 01）. The results showed that both anti-androgen drugs and MGA treatment promoted the development of regrowth antlers and increased the weight of regrowth antlers,w

关 键 词：梅花鹿 抗雄性激素 孕酮 睾酮 二茬茸 

分 类 号：S825[农业科学—畜牧学]