人类白细胞抗原-G基因上游调控区单核苷酸多态性与重度子痫前期发病的关系  

Relationship between upstream regulatory region polymorphism in human leukocyte antigen-G gene and severe preeclampsia

作　　者：琚娟[1] 张展[2] 高峻峻[1] 王宝金[1] Ju Juan;Zhang Zhan;Gao Junjun;Wang Baojin(Department of Gynecology and Obstetrics,the Third Affiliated Hospitalof Zhengzhou University,Zhengzhou 450052,Chin;Zhengzhou University,Zhengzhou 450000,China（Zhang Zhan)

机构地区：[1]郑州大学第三附属医院妇产科,450052 [2]郑州大学,450000

出　　处：《中华微生物学和免疫学杂志》2018年第7期518-522,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（30872316）;河南省科技创新杰出人才基金计划（124200510005）

摘　　要：目的探讨人类白细胞抗原-G（HLA-G）上游调控区单核苷酸多态性与重度子痫前期发病的关系。方法选择2008年10月至2009年3月郑州大学第三附属医院妇产科住院的39例孕晚期重度子痫前期孕妇为病例组，另选择同期正常孕晚期孕妇43例为对照组，两组孕妇均为汉族居民。采用PCR测序技术对两组孕妇进行HLA-G基因上游调控区单核苷酸多态性的等位基因和基因型分析，分别比较两组孕妇间单核苷酸多态性位点的等位基因和基因型频率分布。结果（1）中国汉族孕妇中，HLA-G基因上游调控区－1179～－689位点之间共检测到8个单核苷酸多态性位点；（2）重度子痫前期组孕妇HLA-G基因－964位点基因型GG的频率为12.8%（5/39），显著低于对照组的37.2%（16/43），两组比较差异有统计学意义（P＝0.012）；重度子痫前期组孕妇HLA-G基因－964位点等位基因G的频率为38.5%（30/78），显著低于对照组的57.0%（49/86），两组比较，差异有统计学意义（P＝0.018）；（3）重度子痫前期组孕妇HLA-G －716位点基因型TT的频率为17.9%（7/39），显著低于对照组的41.9%（18/43），两组比较差异有统计学意义（P＝0.019）；重度子痫前期组孕妇HLA-G －716位点等位基因T的频率为39.7%（31/78），显著低于对照组的60.5%（52/86），两组比较差异有统计学意义（P＝0.008）；（4）HLA-G －1140A/T、－762C/T、－725C/G的基因型及等位基因在重度子痫前期组和对照组的分布差异无统计学意义（P〉0.05）。结论中国汉族孕妇中，HLA-G基因上游调控区部分单核苷酸多态性与重度子痫前期的发病有关，－964位点等位基因为G或基因型为GG、－716位点等位基因为T或基因型为TT时，发生重度子痫前期的风险会降低。ObjectiveTo investigate the relationship between the polymorphism of upstream regulatory region （URR） in human leukocyte antigen-G （HLA-G） gene and the pathogenesis of severe preeclampsia.MethodsThirty-nine gravidas with severe preeclampsia, who were admitted to the Third Affiliated Hospital of Zhengzhou University from October 2008 to March 2009, were enrolled as the case group. Another 43 healthy gravidas at the third trimester were chosen as the control group. All gravidas in both groups were Han nationality. URR polymorphism in HLA-G gene was detected by PCR sequencing. Allele frequencies and genotype frequencies of all single nucleotide polymorphisms （SNPs） were compared between the two groups.Results（1） Eight SNPs were detected between -1179 and -689 in HLA-G gene URR in Chinese Han pregnant women. （2） In the severe preeclampsia group, the genotype frequency of -964GG was 12.8% （5/39）, which was significantly lower than the frequency of 37.2% （16/43） found in the control group （P=0.012）. The allele frequency of -964G was also significantly lower in the severe preeclampsia group than in the control group [38.5% （30/78） vs 57.0% （49/86）, P=0.018）. （3） In the severe preeclampsia group, the genotype frequency of -716TT was 17.9% （7/39）, which was significantly lower than the frequency of 41.9% （18/43） found in the control group （P=0.019）. In the severe preeclampsia group, the allele frequency of -716T was 39.7% （31/78）, which was also significantly lower than the frequency of 60.5% （52/86） found in the control group （P=0.008）. （4） No significant differences were found in the allele or genotype frequencies of -1140A/T, -762C/T or -725C/G in HLA-G gene URR between the two groups （P〉0.05）.ConclusionSome of the SNPs in HLA-G gene URR are associated with the susceptibility of severe preeclampsia in Chinese Han gravidas. Pregnant women carrying -964G or -716T may have reduced risk of severe preeclampsia.

关 键 词：子痫前期 人类白细胞抗原-G 上游调控区 单核苷酸多态性 

分 类 号：R714.244[医药卫生—妇产科学]