大黄素对小鼠的免疫调节作用及对脾细胞TNF-α和IL-10表达的影响  

Immunomodulation of emodin and its effect on the expression of TNF-α and IL-10 in splenocytes from mice

作　　者：刘超 高慧婕 朱玉贞 董晓磊 崔巍 王北 LIU Chao;GAO Hui-jie;ZHU Yu-zhen;DONG Xiao-lei;CUI Wei;WANG Bei(Dept.of Basie Medical Sciences,School of Pharmacy,Jining Medical University,Rizhao 27682;Dept.of Medical hnmunology College of Basic Medical Sciences,Qingdao University,Qingdao 266021;College of Biological Science,Jining Medical University,Rizhao 276826,China)

机构地区：[1]济宁医学院药学院基础医学教研室,山东日照276826 [2]青岛大学基础医学院医学免疫学教研室,山东青岛266021 [3]济宁医学院生物科学学院,山东日照276826

出　　处：《基础医学与临床》2018年第9期1298-1302,共5页Basic and Clinical Medicine

基　　金：山东省医药卫生科技发展计划(2017WS338);济宁医学院青年扶持基金(JY2016KJ004Z);济宁医学院博士启动基金(JY2015BS23)

摘　　要：目的探讨大黄素对小鼠的免疫调节作用,以及对免疫炎性因子TNF-α和IL-10表达的影响。方法将ICR小鼠随机分为对照组、环磷酰胺组、大黄素高和低剂量组(10和5 mg/kg)。实验进行7 d,测定各组小鼠胸(脾)指数、血清溶血素含量、外周血T淋巴细胞转化率;ELISA检测外周血TNF-α和IL-10表达水平以及RT-PCR检测脾细胞TNF-α和IL-10 mRNA的表达。结果大黄素降低了小鼠的胸(脾)指数和外周血T淋巴细胞转化率,但提高了小鼠血清溶血素含量;大黄素用药组小鼠外周血TNF-α的表达水平降低,IL-10的表达水平上升;大黄素用药组小鼠脾细胞TNF-αmRNA的表达降低,IL-10 mRNA的表达升高。结论大黄素可以提高机体体液免疫功能,同时表现出良好的抗炎活性,但是对于免疫器官和细胞免疫功能有一定的抑制作用。Objective To investigate the immunomodulatory effects of emodin and its effect on the expression of TNF-α and IL-10 in peripheral blood and splenocytes in mice. Methods ICR mice were randomly divided into control group,cyclophosphamide group,emodin high and low dose（ 10 and 5 mg/kg） group. After 7 days,the thymus and spleen index,the content of serum hemolysin and peripheral blood lymphocyte transformation rate were analyzed. The expression of TNF-α and IL-10 in peripheral blood was detected by ELISA. RT-PCR was used to detect the changes of TNF-α and IL-10 mRNA expression in mouse spleen cells. Results The application of emodin reduced the thoracic spleen index and peripheral lymphocyte transformation rate,but increased the serum hemolysin level in mice. The level of TNF-α in emodin group was decreased and the level of IL-10 was increased. The mRNAexpression of IL-10 in spleen cells of emodin group increased and the expression of TNF-α mRNA decreased.Conclusions Emodin can improve the humoral immune function of mice and exhibit good anti-inflammatory activity,but it has certain inhibitory effect on immune organs and cell immune function.

关 键 词：大黄素 免疫调节 肿瘤坏死因子-α 白细胞介素-10 

分 类 号：R285.5[医药卫生—中药学]