4株新出现的Ⅰ类新城疫病毒F基因的变异分析  

Distribution and Molecular Characterization of 4 New ClassⅠ Newcastle Disease Viruses Isolated from China

作　　者：魏润宇 罗瑶瑶 吕艳 王静静 郑东霞 赵云玲 刘文博[1,3] 刘华雷 王志亮 WEI Run-yu;LUO Yao-yao;LUYan;WANG Jing-jing;ZHENG Dong-xia;ZHAO Yun-ling;LIU Wen-bo;LIU Hua-lei;WANG Zhi-liang(College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;China Animal Health and Epidemiology Center,Qingdao 266032,China;Qingdao Agricultural University,Qingdao 266109,China;A Project Funded by the Priority Academic Program Development of J iangsu Higher Education Institutions,Yangzhou 225009,China)

机构地区：[1]扬州大学兽医学院,扬州225009 [2]中国动物卫生与流行病中心,青岛266032 [3]江苏高校动物重要疫病与人畜共患病防控协同创新中心,扬州225009 [4]青岛农业大学,青岛266109

出　　处：《畜牧兽医学报》2018年第8期1781-1788,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点研发计划(2016YFD0500800);公益性行业(农业)科研专项(201303033);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：为了分析我国新出现的Ⅰ类新城疫病毒的分布和分子特性,对2016年分离到的4株Ⅰ类新城疫病毒进行F基因序列分析和遗传进化分析。结果显示:4株Ⅰ类新城疫病毒F基因编码长度均为1 662nt,共编码553个氨基酸,分离株之间氨基酸相似性为97.9%~100%。4株分离株F蛋白裂解位点的氨基酸组成均为112 ERQERL117,符合新城疫病毒弱毒株的分子特征。F基因高变区(47—420nt)遗传进化分析结果显示这4株病毒为国内新出现的基因型,利用F基因编码区全长序列构建系统进化树显示4株病毒属于基因1c亚型,与北美分离株高度同源,而与国内基因1c亚型分离株(Duck/Guangxi/1261/2015)相似性仅为95.5%~97%。监测数据表明这种新出现的Ⅰ类新城疫病毒具有在国内进一步流行和扩散的风险。In order to understand the distribution and molecular characteristics of new emerged classⅠ Newcastle disease virus in China.We carried out the Fgene sequence analysis and phylogenetic analysis of 4 viruses isolated in 2016.Results were as follows：The length of complete F gene of 4 NDV isolates were 1 662 nt,encoding 553 amino acids,and the amino acid identity among all isolates was 97.9%-100%.The amino acid motif at F protein cleavage site of the 4 isolates was 112 ERQERL117,which was consistent with that of lentogenic Newcastle disease virus.The phylogenetic analysis of Fgene（47-420 nt）showed that all 4 isolates were newly emerging NDVs in China.Phylogenetic tree constructed by the complete Fgenes showed 4 ClassⅠ viruses belonged to sub-genotype 1 c,which showed a high level of similarity with isolates obtained from North America,and had the identity of 95.5%-97% with the sub-genotype 1 cvirus（Duck/Guan-gxi/1261/2015）isolated in China.Our results indicated that the new emerged class Ⅰ NDVs had the ability to circulate and spread in China.

关 键 词：Ⅰ类新城疫病毒 分子特征 遗传进化分析 

分 类 号：S852.659.5[农业科学—基础兽医学]