山羊疱疹病毒Ⅰ型的分离鉴定与致病性  

Identification and Pathogenicity of One Caprine Herpesvirus 1Strain

作　　者：郝飞[1] 毛立[1] 李文良[1] 李基棕[1] 杨蕾蕾[1] 张纹纹[1] 孙敏 钟纯燕 刘茂军[1] 江杰元[1] HAO Fei;MAO Li;LI Wen-liang;LI Ji-zong;YANG Lei-lei;ZHANG Wen-wen;SUN Min;ZHONG Chun-yan;LIU Mao-jun;JIANG Jie-yuan(Key Laboratory for Veterinary Bio-Product Engineering of Ministry of Agriculture,Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;College of Animal Science,Guizhou University,Guiyang 550025,China)

机构地区：[1]江苏省农业科学院兽医研究所,农业部兽用生物制品工程重点实验室,南京210014 [2]贵州大学动物科学学院,贵阳550025

出　　处：《畜牧兽医学报》2018年第8期1797-1802,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：“十三五”国家重点研发计划(2016YFD0500900); 江苏省农业科技自主创新资金项目(CX(14)2090)

摘　　要：为了查明引起2013—2014年江苏多地育肥羊出现呼吸道症状为主的疾病的病因,通过采集发病羊场鼻拭子,采用细胞接毒分离培养、病毒蚀斑纯化、病毒形态结构观察和病毒核酸鉴定,分离到一株山羊疱疹病毒Ⅰ型(caprine herpesvirus 1,CpHV-1),命名为JSHA1405;并对其进行了致病性试验。分离鉴定结果表明:病料接种MDBK细胞后,盲传至第3代时产生明显细胞病变;电镜下可见直径约100nm有囊膜的病毒粒子;gB全基因测序表明其为CpHV-1,序列比对显示JSHA1405株与CpHV-1瑞士分离毒E/CH株相似性最高,为99.2%。致病性试验表明,JSHA1405分离株可导致山羊发热持续一周以上,体温最高可达41.8℃。山羊感染后临床症状明显,表现为精神沉郁、流浆液性或脓性鼻涕,可通过鼻腔/粪便排毒。山羊感染后第7天出现中和抗体,第28天达到最高。这是国内首次报道CpHV-1的分离鉴定及致病性,分离毒JSHA1405具有较强的致病性,为CpHV-1病原学及防控技术研究提供依据与参考。This study aimed to find the cause of severe respiratory disease in fattened goat herds of many farms in Jiangsu province during 2013 to 2014.In this study,one caprine herpesvirus 1 named JSHA1405 was detected and isolated from nasal swab samples of goat herds with severe respiratory disease,and identified by transmission electron microscopy,PCR and virus pathogenicity test.MDBK cells infected with the third generation of JSHA1405 showed typical cytopathogenic effect（CPE）.Under transmission electron microscopy detection,the enveloped virion could be observed with the diameter of 100 nm.Sequence analysis of gB gene indicated that JSHA1405 had some variation compared with E/CH strain,and the homology was 99.2%.Furthermore,the pathogenicity test indicated that JSHA1405 could induce high fever（peaked at41.8 ℃）and lasted for about one week,other symptoms included depression,nasal discharge.Virus shedding could be detected from nasal and rectal swabs.Neutralizing antibodies appearedfrom the 7 th day post infection（dpi）and peaked at 28 th dpi.This is the first report about isolation,identification and pathogenicity study of CpHV-1 in China.The experiments demonstrated that the CpHV-1 strain JSHA1405 was pathogenic to goats.It provides foundation for further study of the viral etiology and the prevention and control of CpHV-1.

关 键 词：山羊疱疹病毒Ⅰ型 分离鉴定 致病性 GB 

分 类 号：S852.659.1[农业科学—基础兽医学]