甘乐片对大鼠酒精肝损伤的保护作用及机制研究  

Protective effect and mechanism of Gan Le Tablet on alcohol hepatic injury in rats

作　　者：李海龙 周勇 薛照芸[2] 黄光业 石硕 陈健文[2] LI Hai-long;ZHOU Yong;XUE Zhao-yun;HUANG Guang-ye;SHI Shuo;CHEN Jian-wen(Infinitus R ＆ D Center,Guangzhou,Guangdong 510663;School of Pharmaceutical Science,Sun Yat-sen University,Guangzhou,Guangdong 510006,China)

机构地区：[1]无限极研发中心,广东广州510663 [2]中山大学药学院,广东广州510006

出　　处：《热带医学杂志》2018年第8期1032-1035,共4页Journal of Tropical Medicine

基　　金：广东省中药局基金项目(20151167)

摘　　要：目的考察甘乐片对酒精肝损伤的保护作用及机制。方法 60只SD大鼠分为正常组、模型组、甘乐片低剂量组(300 mg/kg)、甘乐片中剂量组(600 mg/kg)、甘乐片高剂量组(1 200 mg/kg)、联苯双酯组(5 mg/kg)。每日除正常组外各组大鼠口服给予60%乙醇溶液10 m L/kg,6 h后口服给予相应的药物,连续给药30 d。末次给药后禁食16 h,取血及肝组织。测定血清中乳酸脱氢酶(LDH)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、一氧化氮合酶(NOS)、一氧化氮(NO)的水平;测定肝组织中丙二醛(MDA)、谷胱甘肽(GSH)、甘油三酯(TG)的水平及引物Bcl-2、Bax、Caspase-3的m RNA表达。结果甘乐片中、高剂量组可显著降低酒精肝损伤大鼠血清LDH、AST、ALT、NOS、NO水平;降低肝组织MDA、TG,提高GSH;提高Bcl-2 m RNA的表达,降低Bax和Caspase-3 m RNA的表达,与模型组比较,差异均有统计学意义(P<0.05)。结论甘乐片中、高剂量对实验性酒精肝损伤具有良好的保护作用,其作用机制可能与减少过氧化损伤,降低NOS、NO水平,调节Bcl-2、Bax和Caspase-3的表达有关。Objective To investigate the protective effect and mechanism of Gan Le Tablet（GLT） on alcoholic liver injury.Methods 60 male SD rats were randomly divided into 6 groups： normal control group, model group, GLT groups（300,600,1 200 mg/kg）, bifendate group（5 mg/kg）. For 30 days, except the normal control group, the other groups were given60% ethanol solution orally 10 m L/kg, and given prescribed drug treatments after 6 hours. After the above procedures, thelevels of lactate dehydrogenase（LDH）, aspartate transaminase（AST）, alanine aminotransferase（ALT）, nitric oxide synthase（NOS）, nitric oxide（NO）in serum, and Malondialdehyde（MDA）, glutathione（GSH）, triglyceride（TG）in liver, and them RNA expression of Bcl-2, Bax, Caspase-3 in liver were measured accordingly. Results GLT（600, 1200 mg/kg） couldreduce the serum LDH, AST, ALT, NOS, NO and hepatic MDA, TG while increase hepatic GSH. GLT also could increasethe m RNA expression of Bcl-2 while reduce m RNA expression of Bax and Caspase-3.The difference was statisticallysignificant（P〈0.05）. Conclusion GLT（600, 1200 mg/kg） had favorable anti-effects on alcoholic liver injury. Its possiblemechanisms might be related to reducing oxidative damage and the levels of NOS and NO, and regulating the expression ofBcl-2,Bax and Caspase-3.

关 键 词：甘乐片 酒精肝损伤 氧化损伤 凋亡 

分 类 号：R96[医药卫生—药理学]