双辛鼻鼽散对过敏性鼻炎大鼠血清及鼻腔灌洗液细胞因子的影响  

Effect of Shuangxin Biqiu San(双辛鼻鼽散, SXBQS) on Cytokines in Serum and Nasal Fluid Lavage of Allergic Rhinitis Rats

作　　者：曹鹏 赛佳洋 屈会化 孔慧 王雪茜 王庆国 赵琰 CAO Peng;SAI Jia-yang;QU Hui-hua;KONG Hui;WANG Xue-qian;WANG Qing-guo;ZHAO Yan(School of Preelinlcal Medicine,Beifing University of Chinese Medicine,Beijing 100029,China;The Third Affiliated Hospital,Beifing University of Chinese Medicine,Beijing 100029,China;Center of Scientific Experiment,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学中医学院,北京100029 [2]北京中医药大学第三附属医院,北京100029 [3]北京中医药大学北京中医药研究院,北京100029

出　　处：《中医药导报》2018年第16期61-64,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京市科学技术委员会十病十药研发-双辛鼻鼽散治疗过敏性鼻炎的成药性研究(Z151100003815017);双辛鼻鼽散合作研究协议(2010072120009)

摘　　要：目的:研究双辛鼻鼽散治疗过敏性鼻炎的免疫调节机制。方法:建立卵清蛋白(OVA)诱导的BN大鼠AR模型,随机分为正常组、模型组、盐酸西替利嗪组及双辛鼻鼽散高、中、低剂量组。药物干预3周,检测各组大鼠血清中IFNγ、IL-4、IL-5、IL-17及鼻腔灌洗液中Ig E、组胺、IL-4含量。结果:双辛鼻鼽散各剂量组及盐酸西替利嗪组不同程度上调血清IFNγ、下调血清IL-4、IL-17水平(P<0.05);双辛鼻鼽散高剂量组可显著下调IL-5表达(P<0.05)。双辛鼻鼽散高剂量及盐酸西替利嗪组显著降低大鼠鼻腔灌洗液总蛋白浓度(P<0.05);双辛鼻鼽散高、中剂量及盐酸西替利嗪Ig E含量低于模型组(P<0.05);各组大鼠鼻腔灌洗液组胺含量比较,差异无统计学意义(P>0.05);各给药组IL-4水平有不用程度降低,双辛鼻鼽散高剂量组与模型组比较,差异有统计学意义(P<0.05)。结论:双辛鼻鼽散可通过调控T细胞因子进而起到免疫调节作用。Objective： To study the immunoregulation mechanism of SXBQS. Methods： BN rats allergic to o- valbumin （OVA） were prepared, and the rats were divided into 6 groups, including model group, CTRZ group, SXBQS-high group, SXBQS-mid group, SXBQS-low group and control group. IFNγ IL-4, IL-5, IL-17, IgE and histamine in serum and nasal fluid lavage were determined after three-week treatment. Results： Serum indexes of IL-4, IL-17 levels were lower in SXBQS treated group than those from the OVA-induced group, while IFNγ secretion was up regulated by SXBQS （P〈0.05）. SXBQS （high） treatment significantly decreased serum IL-5 level in serum（P〈0.05）. CTRZ and SXBQS （high） treatment decreased concentration of total protein in nasal secretion （P〈0.05）. IgE, IL-4 levels in nasal perfusate were downregulated by SXBQS （P〈0.05）. There were no differences of histamine levees in nasal perfusate among the six groups （P〉0.05）. Conclusion： SXBQS exerts immunoregulated properties by regulating T cell related cytokines both in serum and nasal lavage.

关 键 词：过敏性鼻炎 双辛鼻鼽散 T细胞 免疫调节 鼻腔灌洗液 细胞因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]