人外周血单个核细胞与肝细胞HBV受体蛋白的表达  

The expression of HBV receptor protein in human peripheral blood monocytes(PBMCs)and hepatocytes

作　　者：田景惠[1] 林少为 唐丽华[2] 李冬[1] 陈强[1] 李宝华[1] 徐希柱[1] 田兆菊[1] 焦凤萍[1] TIAN Jing-hui;LIN Shao-wei;TANG Li-hua;LI Dong;CHEN Qiang;LI Bao-hua;XU Xi-zhu;TIAN Zhao-ju;JIAO Feng-ping(Department of Clinical School of Public Health,Taishan Medi-cal University,Taian,Shandong 271000,China;Department of Blood Transfusion,Taian City Central Hospital)

机构地区：[1]泰山医学院公共卫生学院,山东泰安271000 [2]泰安市中心医院输血科

出　　处：《中国病原生物学杂志》2018年第7期691-693,共3页Journal of Pathogen Biology

基　　金：国家自然科学基金项目(No.31500148);泰安市科技发展计划资助项目(No.2015NS1131;201440774-13);山东省自然科学基金项目(No.ZR2012HM082)

摘　　要：目的检测乙型肝炎病毒(HBV)受体蛋白在人外周血单个核细胞(PBMC)与肝细胞的表达情况,探讨HBV进入PBMC的可能途径,以期了解HBV感染PBMC的可能机制。方法用淋巴细胞分离液分离人PBMC。用RIPA提取人PBMC和肝细胞(肝脏肿瘤癌旁组织)总蛋白,采用Western blot以检测HBV受体蛋白在两种细胞的表达情况。结果 Western blot检测肝细胞HBV受体蛋白阳性,与hNTCP抗体反应条带位于(35~40)×103之间,与预期一致;人PBMC蛋白无此反应条带,即hNTCP检测阴性。结论 PBMC不表达肝细胞表面的HBV受体蛋白,病毒可能循非受体途径进入PBMC,或PBMC表面的HBV受体与肝细胞表面的HBV蛋白受体蛋白不同。关于HBV感染PBMC的具体机制尚需进一步研究。Objectives To explore the possible pathways by which hepatitis B virus（HBV）enters peripheral blood monocytes（PBMCs）and to understand the possible mechanisms by which HBV infects PBMCs by detecting the expression of HBV receptor in PBMCs and hepatocytes. Methods In order to detect the expression of HBV receptor in PBMCs and hepatocytes,PBMCs were isolated with Ficoll-Hypaque.Total protein from PBMCs and hepatocytes from liver tumor-adjacent tissues was extracted with RIPA and then assayed using a Western blot assay. Results A Western blot assay indicated that HBV receptor protein expression was positive in hepatocytes,and the reaction band with the hNTCP antibody was located between（35~40）×103,as expected.However,the same reaction band with the hNTCP antibody was not detected in PBMCs,and HBV receptor protein expression was negative. Conclusion PBMCs do not express HBV receptor protein,though it was found on the surface of hepatocytes,suggesting that HBV may enter PBMCs via non-receptor pathways or that HBV receptor on the surface of PBMCs differs from HBV receptor on the surface of hepatocytes.More research is needed to interpret the exact mechanism by which HBV infects PBMCs.

关 键 词：乙型肝炎病毒 外周血单个核细胞 受体 Na+-牛磺胆酸共转运蛋白 

分 类 号：R373.21[医药卫生—病原生物学]