云南省HIV-1新近感染人群中病毒辅助受体流行情况分析  

The prevalence of HIV-1 co-receptor usage among the recently infected population in Yunnan Province

作　　者：陈敏[1] 陈会超[1] 戴洁[1] 杨朝军[1] 董莉娟[1] 杨莉[1] 杨敏 马艳玲[1] CHEN Min;CHEN Hui-ehao;DAI Jie;YANG Chao-jun;DONG Li-juan;YANG Li;YANG Min;MA Yan-ling(Institute for A IDS/STDs Control and Prevention,Yunnan Provincial Center for Disease Control and Prevention,Kunming,Yunnan 650022,China)

机构地区：[1]云南省疾病预防控制中心性病艾滋病防制所,云南昆明650022

出　　处：《现代预防医学》2018年第16期2881-2884,2890,共5页Modern Preventive Medicine

基　　金：云南省运用基础研究计划项目(2015FB200);国家自然科学基金项目(81560327)

摘　　要：目的研究云南省HIV-1新近感染人群中病毒辅助受体的流行情况。方法收集云南省2014年1-3月和2015年1-6月新报告HIV-1感染者的血浆样品9 185份,对BED-捕获酶免疫试验判断为新近感染的样品进行env基因扩增和基因分型,用Geno2Phemo和Web PSSM对病毒使用的辅助受体进行分析。结果 933份样品被判断为新近感染,扩增后获得546条env序列,分型包括C亚型(81.9%),CRF01_AE(17.2%),B亚型(0.7%)和CRF01_AE/C(0.2%)。异性性接触和注射吸毒感染中以C亚型为主,分别占86.7%和87.9%,而同性性接触感染中以CRF01_AE为主,占51.5%(34/66)。不同CD4+T淋巴细胞计数分组中,病毒基因型的构成无统计学差异。研究对象中,CCR5嗜性病毒占90.3%,CXCR4嗜性病毒占9.7%。CRF01_AE中CXCR4嗜性病毒的比例(45.7%)高于C亚型(2.0%)。同性性接触传播中CXCR4嗜性病毒的比例为27.3%,高于异性性接触和注射吸毒传播(7.5%和6.5%)。不同CD4+T淋巴细胞计数的分组中,CCR5和CXCR4嗜性病毒的构成无统计学差异。结论新近感染人群中存在着一定比例的CXCR4嗜性的病毒,应加强对流行株的细胞嗜性的监测和评估。Objective To investigate the prevalence of HIV-1 co-receptor usage among the recently infected population in Yunnan Province. Methods A total of 9 186 plasma samples were collected from the newly reported HIV-1 infectors in Yunnan Province from January to March 2014 and January to June 2015. The samples identified as recent infections with BED captured enzyme immunoassay（CEIA） were used for env gene amplifying.Viral co-receptor usage was analyzed with two online tools： Geno2 Pheno and Web PSSM. Results A total of 933 samples were identified as recent infections, from which 546 env sequences were successfully amplified. Based on env sequences, HIV-1 genotypes included subtype C（81.9%, 447/546）,CRF01_AE（17.2%, 94/546）, subtype B（0.7%, 4/546） and CRF01_AE/C（0.2%, 1/546）. Subtype C was the predominant strain in heterosexual contact and intravenous drug use, accounted for 86.7%（447/546） and 87.9%（40/46）. CRF01_AE was the predominant strain in heterosexual contact, accounted for 51.5%（34/66）. The constituent ratios of HIV-1 subtypes showed no statistically difference among the groups stratified by CD4 cells counting. Among these subjects, CCR5 tropic viruses（only using CCR5 as co-receptor） accounted for 90.3%（493/546）, CXCR4 tropic viruses（using CXCR4 or CXCR4/CCR5 as co-receptor） accounted for 9.7%（53/546）. The proportion of CXCR4-tripic viruses among CRF01_AE linage（45.7%%, 43/94）was significantly higher than that among subtype C linage（2.0%, 9/447）. The proportion of CXCR4 tropic viruses among homosexual contact（27.3%, 18/66） was statistically higher than those among heterosexual contact（7.5%%, 32/427） and drug use（6.5%, 3/46）. The constituent ratios of CCR5 tropic viruses and CXCR4 tropic viruses showed no statistically difference among the groups stratified by CD4 cells counting. Conclusion A certain proportion of CXCR4 tropic viruses exist in newly infected populations, and monitoring and evaluation of cell tropism of epidemic strains should be

关 键 词：人类免疫缺陷病毒-1 新近感染 辅助受体 

分 类 号：R181.2[医药卫生—流行病学]