卡介菌多糖对胰腺癌细胞系增殖及凋亡影响的研究  

Effect of polysaccharide purified from Bacillus Calmette-Guerin formulation on proliferation and apoptosis of pancreatic cancer cell lines

作　　者：吴翔[1] 高戈[2] 卓书伟 WU Xiang;GAO Ge;ZHUO Shu-wei(Hainan Provincial Hospital of Traditional Chinese Medicine,Haikou,Hainan 570203,Chin)

机构地区：[1]海南省中医院,海南海口570203 [2]中南大学湘雅医学院检验系

出　　处：《实用预防医学》2018年第9期1046-1049,共4页Practical Preventive Medicine

基　　金：海南省自然科学基金面上项目(818MS159)

摘　　要：目的探讨卡介菌多糖对胰腺癌细胞系增殖和抗凋亡机制。方法 3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide,MTT)比色法MTT法检测卡介菌多糖对胰腺癌PANC-1、SW1990、原代细胞的增殖抑制作用;流式细胞术检测卡介菌多糖对胰腺癌PANC-1、SW1990、原代细胞的凋亡率。结果高、中、低浓度的卡介菌多糖组对PANC-1、SW1990、原代细胞增殖抑制率(高浓度分别为:9.53±0.82%、10.02±0.95%、9.08±0.78%;中浓度分别为:8.84±0.77%、9.62±0.95%、8.18±0.71%;低浓度分别为:5.72±0.48%、6.48±0.48%、5.06±0.44%)明显高于空白对照组的3.92±0.28%、4.72±0.40%、2.91±0.26%和卡介菌多糖核酸组的4.82±0.48%、5.46±0.46%、4.22±0.38%;卡介菌多糖与吉西他滨联用对PANC-1、SW1990、原代细胞增殖抑制率分别为16.54±1.56%、18.28±1.86%、15.88±1.92%,优于GEM或卡介菌多糖单独使用,也高于卡介菌多糖核酸与吉西他滨联用的13.82±1.42%、14.88±1.72%、12.32±1.18%,其差异有统计学意义(均P<0.05)。在对PANC-1、SW1990、原代细胞凋亡率方面同样高、中、低浓度的卡介菌多糖组明显高于空白对照组和卡介菌多糖核酸组,卡介菌多糖与GEM联用,优于GEM或卡介菌多糖单独使用,也高于卡介菌多糖核酸与GEM联用,差异均有统计学意义(P<0.05)。结论卡介菌多糖具有抗肿瘤作用,其作用效果优于卡介菌多糖核酸,与吉西他滨联合作用效果更显著。Objective To explore the mechanism of polysaccharide purified from Bacillus Calmette-Guerin（ BCG） formulation on proliferation and anti-apoptosis of pancreatic cancer cell lines. Methods The inhibitory effect of polysaccharide purified from BCG formulation on cell growth of pancreatic cancer cell line PANC-1,SW1990 and primary cells was detected by 3-（ 4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide（ MTT） assay. Flow cytometry was used to detect the cell apoptosis rates. Results The inhibitory rates of PANC-1,SW1990 and primary cell proliferation were respectively（ 9. 53 ± 0. 82） %,（10. 02± 0.95）%,（9.08± 0.78）% in the high-concentration group of purified polysaccharide from BCG formulation,（8.84±0. 77） %,（ 9.62±0.95） %,（ 8.18±0.71） % in the medium-concentration group,and（ 5.72± 0.48） %,（ 6.48± 0.48） %,（ 5.06±0. 44） % in the low-concentration group,which were all significantly higher than those of the blank control group（（ 3.92±0.28） %,（4.72±0.40）%,（2.91± 0.26）%） and BCG-polysaccharide nucleic acid group（（4.82± 0.48）%,（5.46± 0.46）%,（4.22±0. 38） %）. The inhibitory rates of PANC-1,SW1990 and primary cell proliferation in the group of purified polysaccharide combined with gemcitabine（ GEM） were（ 16.54±1.56） %,（ 18.28±1.86） % and（ 15.88±1.92） % respectively,which were superior to those of GEM or purified polysaccharide alone group as well as those of BCG-polysaccharide nucleic acid plus GEM group（（ 13. 82 ±1. 42） %,（ 14. 88 ± 1. 72） %,（ 12. 32 ± 1. 18） %）,with statistically significant differences（ all P〈0. 05）. The apoptosis rates of PANC-1,SW1990 and primary cell proliferation were significantly higher in the groups of high-,medium-and low-concentration of purified polysaccharide than in the blank control group and BCG-polysaccharide nucleic acid group. The apoptosis rates of PANC-1,SW1990 and primary cell in the group of purified polysaccharide combined with GEM w

关 键 词：卡介菌多糖 胰腺癌 增殖 凋亡 

分 类 号：R392.1[医药卫生—免疫学]