金蝉口服液中黄芩苷的HPLC法含量测定  

Determination of Baicalin in Jinchankoufu Liquor by HPLC

作　　者：金慧臻[1] 黄艳[1] 毛晨梅[1] 吕承哲[1] 孟永钦[1] 郁丹红[1] JIN Hui-zhen;HUANG Yan;MAO Chen-mei;Lü Cheng-zhe;MENG Yong-qin;Yu Dan-hong(Children＇s Hospital Affiliated to Suzhou University,Suzhou Jiangsu 215000,China)

机构地区：[1]苏州大学附属儿童医院,江苏苏州215000

出　　处：《抗感染药学》2018年第6期937-939,共3页Anti-infection Pharmacy

基　　金：江苏省药学会-奥赛康临床药学基金科研项目(编号:201510)

摘　　要：目的:建立HPLC法测定金蝉口服液中黄芩苷含量的方法。方法:采用色谱柱为Nucleosil C_(18)柱(250 mm×4.6 mm,5μm);流动相为甲醇-水-磷酸-三乙胺(48∶52∶0.4∶0.4),体积流量为1.0 m L/min;检测波长为280 nm,柱温为25℃。结果:在该液相条件下黄芩苷成分能很好的达到基线分离,有较宽的线性范围和良好的线性关系,平均加样回收率为102.3%,平均含量为3.295 mg/m L。结论:本法准确、灵敏、可靠、重复性好,可用于金蝉口服液的质量控制。Objective： To establish the method for determining of the content of baicalin in the Jinchankoufu liquor by HPLC. Methods： The chromatographic separation was achieved on a Lichrospher C_（18）（4.6 mm × 250 mm,5 μm） column with a mobile phase composed of methanol-water-phosphoric acid-triethylamine（48：52：0.4：0.4）. The flow rate was 1.0 m L/min, the column temperature was at 25 ℃ and the detection wave length was set at 280 nm. Results： The results showed that baicalin was separated well and showed good linearity. The average recoveries were 102.3%. The average content of baicalin was 3.295 mg/m L. Conclusion： The method is proved to be accurate, sensitive, credible and reliable. It can be applied to the quality control of compound prepartion of Jinchankoufu liquor.

关 键 词：HPLC 金蝉口服液 质量标准 黄芩苷 

分 类 号：R969.3[医药卫生—药理学]