Homer在2型糖尿病大鼠海马中的表达及意义的研究  

Homer expression in the hippoeampus of rats with type 2 diabetes mellitus

作　　者：方士强 罗振钊[1] 李晓怡 刘水逸[1] 卢忠心[1] FANG Shiqiang, LUO Zhenzhao ,LI Xiaoyi ,et al.(Department of Medical Laboratory, The Central Hospital of Wuhan , Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430014 ,China)

机构地区：[1]华中科技大学同济医学院附属武汉中心医院检验科,武汉430014

出　　处：《中国糖尿病杂志》2018年第8期676-679,共4页Chinese Journal of Diabetes

基　　金：武汉市卫计委临床医学科研项目(WX13C09)

摘　　要：目的评价Homer蛋白在T2DM大鼠海马中的表达及意义,并分析其与T2DM大鼠认知障碍间的关系。方法糖尿病大鼠模型组(T2DM组)、对照(Con)组大鼠各13只。取T2DM组及Con组各8只大鼠的海马组织,采用RT-qPCR法检测大鼠海马组织中Homer1c、Homer2、Homer3mRNA的表达。取两组另外各5只大鼠海马组织,Western blot法检测上述基因蛋白表达是否存在差异。结果T2DM组海马组织Homerlc[(29.99±5.11)vs(19.91±4.08)]、Homer2[(26.62±4.14)vs(15.79±2.58)]和Homer3[(35.08±3.27)vs(16.16±2.49)]的mRNA水平高于Con组(P<0.05或P<0.01);T2DM组海马组织Homerlc[(8.4±0.9)vs(3.2±0.5)]、Homer2[(6.8±0.6)vs(1.5±0.3)]和Homer3[(2.4±0.3)vs(0.9±0.2)]蛋白水平高于Con组(P<0.05)。结论Homerlc、Homer2和Homer3可能参与T2DM认知障碍,为糖尿病脑病提供新的实验依据和潜在的治疗靶点。Objective To investigate the expressions of Homer proteins in hippoeampus of rats with type 2 diabetes mellitus（T2DM）,and discuss the relationship between Homer and cognitive dysfunction in rats with T2DM. Methods A total of 13 rats with T2DM and 13 normal rats were enrolled in this study.The hippocampus was dissected from 8 rats with T2DM and 8 normal rats.The differential expressions of Homerlc,Homer 2,Homer 3 mRNA were measured by RT-qPCR.Protein concentrations of hippocampus of the left 5 rats in each group were isolaled.The expression of the above genes＇ protein which were found expressed differentially on mRNA level were tested by Western blot. Results The mRNAs of Homerlc[（29.99±5.11）vs（19.91±4.08）],Homer2[（26.62±4.14）vs（15.79±2.58）]and Homer 3[（35.08±3.27）vs（16.16±2.49）]genes were significantly higher in rats with T2DM than in Con group（P〈0.05 or P〈0.01）.The levels of Homerlc[（8.4±0.9）vs（3.2±0.5）],Homer 2[（6.8±0.6）vs（1.5±0.3）]and Homer3[（2.4±0.3）vs（0.9±0.2）]proteins were also significantly higher rats with T2DM than in Con group（P〈0.05）. Conclusion Homerlc,Homer 2,and Homer 3 may play an important role in cognitive dysfunction of T2DM,which may provide new evidence in diabetic encephalopathy research and targets of therapy.

关 键 词：糖尿病 2型 认知障碍 HOMER 海马 

分 类 号：R587.1[医药卫生—内分泌]