残黄片对免疫低下小鼠免疫调节作用的研究  

Study on Immunoregulation Effect of Residual Huang Tablet on Mice

作　　者：李娜娜 王雯颍 李承平 邓莉[1] 石金金[1] 陈慧芳[3] 孙曼[4] 何永侠 李寅超[1] 袁海龙[6] 韩晋[7] LI Na-na;WANG Wen-ying;LI Cheng-ping;DENG Li;SHI Jin-jin;CHEN Hui-fang;SUN Man;HE Yong-xia;LI Yin-chao;YUAN Hal-long;HAN Jin(School of Pharmaceutcal Sciences of Zhengzhou University,Zhengzhou,450001,China;College of Life Sci-ences of Zhengzhou University,Zhengzhou,450001,China;First Affiated Hospital of Xinxiang Medical Col-lege,Henan Xinxiong,453100,China;Zhengzhou First People＇s Hospital,Zhengzhou,450001,China;Luo Yang Polytechnic School,Luoyang,471000,China;Ceneral Hospital of the Air Force of the Chinese PLA,Bei-ring 100036,China;Department of Pharmacy,Hospital 302 of PLA,Beijing 100039,China)

机构地区：[1]郑州大学药学院,河南郑州450001 [2]郑州大学生命科学学院,河南郑州450001 [3]新乡医学院第一附属医院,河南新乡453100 [4]郑州市第一人民医院,河南郑州450001 [5]洛阳职业技术学院,河南洛阳471000 [6]中国人民解放军空军总医院,北京100036 [7]中国人民解放军第302医院,北京100039

出　　处：《时珍国医国药》2018年第5期1088-1090,共3页Lishizhen Medicine and Materia Medica Research

基　　金：河南省中医药科学研究专项课题(2014ZY02017); 河南省科技计划项目(152102310072); 中国肝炎防治基金会-王宝恩肝纤维化研究基金资助课题(WEB20170066)

摘　　要：目的研究残黄片对免疫低下小鼠非特异性免疫、体液免疫以及细胞免疫调节作用的影响。方法通过腹腔注射环磷酰胺(CXT)建立免疫功能低下小鼠模型,用碳粒廓清试验检测残黄片对免疫低下小鼠非特异免疫功能的影响;采用鸡红细胞免疫试验检测残黄片对免疫低下小鼠体液免疫功能的影响;运用1%DNFB诱导迟发型超敏反应试验检测残黄片对免疫低下小鼠细胞免疫功能的影响。结果在非特异性免疫方面,与模型对照组比较,残黄片中剂量组能显著增加免疫低下小鼠单核巨噬细胞的吞噬能力(P<0.05);在体液免疫方面,与模型对照组比较,残黄片高剂量组可显著升高免疫低下小鼠血清溶血素含量(P<0.05);在细胞免疫方面,未观察到残黄片对免疫低下小鼠的耳肿胀度、耳肿胀率较为明显的影响。结论残黄片对环磷酰胺介导的免疫低下小鼠具有非特异性免疫和体液免疫增强作用,但对细胞免疫功能可能无作用或调节作用较弱。Objective To study the effects of residual yellow tablets on nonspecific immunity,humoral immunity and cellular immune regulation in immunocompromised mice,and to provide experimental evidence for the further research and development of residual tablets. Methods The mice model of immunocompromised mice were established by intraperitoneal injection of cyclophosphamide（ CXT）. The effect of residual yellow tablets on the humoral immunity of immunocompromised mice was detected by chicken erythrocyte immunoassay. The effect of 1% DNFB on delayed hypersensitivity test was used to detect residual effects of yellow tablets on cellular immunity in immunocompromised mice. Results Compared with the model control group,the remnant group could significantly increase the phagocytosis of mononuclear macrophages in immunocompromised mice（ P 〈0. 05）; In humoral immunity,compared with the model control group,high dose group residues yellow tablets can be significantly increased serum hemolysin lower immunized mice（ P 〈0. 05）; In the case of cellular immunity,no residual effect of residual yellow tablets on ear swelling and ear swelling was observed in immunocompromised mice. Conclusion Residual yellow tablets on immunocompromised mice with non-specific immune and humoral immune enhancement,but no effect on cellular immune function or regulation is weak.

关 键 词：残黄片 环磷酰胺 免疫调节 

分 类 号：R285.5[医药卫生—中药学]