外阴阴道假丝酵母菌病患者阴道灌洗液中细胞因子的表达  

Expression of cytokines in vaginal lavage fluid of patients with vulvovaginal candidiasis

作　　者：张永科 谢君 法萍萍 ZHANG Yong-ke;XIE Jun;FA Ping-ping(Department of Obstetrics ＆ Gynycology,Peking University Shenzhen Hospital,Shenzhen Key Labroatory of Gynecological Diagnostic Technology Research,Shenzhen 518036,China)

机构地区：[1]北京大学深圳医院妇产科,518036 [2]深圳市女性重大疾病早期诊断技术重点实验室,518036 [3]北京大学深圳医院中心实验室,518036

出　　处：《中国实用医药》2018年第22期36-38,共3页China Practical Medicine

基　　金：2018年深圳市卫生计生系统科研项目(项目编号:SZXJ2018080)

摘　　要：目的检测并分析阴道灌洗液中白介素-2(IL-2)、白介素-6(IL-6)、白介素-8(IL-8)等细胞因子的浓度,探讨局部免疫因素在外阴阴道假丝酵母菌病(VVC)及复发性外阴阴道假丝酵母菌病(RVVC)发病中的作用。方法选取育龄期在妇科门诊就诊的34例VVC患者作为VVC组,12例RVVC患者作为RVVC组,同期育龄期健康体检女性50例作为对照组。采用酶联免疫吸附试验法(ELISA)对三组阴道灌洗液中IL-2、IL-6、IL-8的浓度进行检测,并进行比较。结果 VVC组、RVVC组IL-2浓度分别为(6.24±0.45)、(6.30±1.62)pg/ml,均低于对照组的(8.12±0.23)pg/ml,差异具有统计学意义(P<0.05)。VVC组、RVVC组IL-8浓度分别为(8.31±0.23)、(8.55±0.49)pg/ml,均高于对照组的(5.92±1.02)pg/ml,差异具有统计学意义(P<0.05)。VVC组、RVVC组IL-6浓度分别为(0.86±0.02)、(0.95±0.11)pg/ml,与对照组的(0.74±0.17)pg/ml比较差异无统计学意义(P>0.05)。结论 RVVC与VVC患者存在阴道局部免疫失衡,调节阴道内免疫稳态可能对治疗该两种疾病有积极意义。Objective To detect and analyze the concentrations of interleukin-2（IL-2）, interleukin-6（IL-6）, interleukin-8（IL-8） and other cytokines in vaginal lavage fluid, and in order to investigate the role of local immune factors in the pathogenesis of vulvovaginal candidiasis（VVC） and recurrent vulvovaginal candidiasis（RVVC）. Methods A total of 34 patients with VVC in child-bearing age in gynecology clinic were selected as VVC group, and 12 RVVC patients were selected as RVVC group. Other 50 healthy women in childbearing age at the same period were selected as control group. Enzyme linked immunosorbent assay（ELISA） was used to detect and compare the concentrations of IL-2, IL-6 and IL-8 of vaginal lavage fluid among the three groups. Results The concentrations of IL-2 in the VVC group and RVVC group were（6.24±0.45） pg/ml and（6.30±1.62） pg/ml respectively, which were significantly lower than（8.12±0.23） pg/ml in the control group, the differences were statistically significant（P0.05）. The concentrations of IL-8 in the VVC group and RVVC group were（8.31±0.23） pg/ml and（8.55±0.49） pg/ml respectively, which were significantly higher than（5.92± 1.02） pg/ml in the control group, the differences were statistically significant（P0.05）. The concentrations of IL-6 in the VVC group and RVVC group were（0.86±0.02） pg/ml and（0.95±0.11） pg/ml respectively, compared with（0.74±0.17） pg/ml in the control group, the differences were not statistically significant（P0.05）. Conclusion The imbalance of vaginal local immunity exists in patients with RVVC and VVC. The regulation of intravaginal immune homeostasis may have positive significances in the treatment of these two diseases.

关 键 词：外阴阴道假丝酵母菌病 复发性外阴阴道假丝酵母菌病 阴道灌洗液 细胞因子 

分 类 号：R711.3[医药卫生—妇产科学]