Expanding the Scope of CRISPR/Cpfl-Mediatec Senome Editing in Rice  

作　　者：Shaoya Li Xin Zhang Wensheng Wang Xiuping Guo Zhichao wu Wenming Du Yunde Zhao Lanqin Xia 

机构地区：[1]Institute of Crop Sciences (ICS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China [2]National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China [3]Section of Ceil and Developmental Biology, University of California, San Diego, La Jolla, CA 92093-0116, USA [4]These authors contributed equally to this article.

出　　处：《Molecular Plant》2018年第7期995-998,共4页分子植物（英文版）

摘　　要：Dear Editor The CRISPR/Cas gene editing system offers great potential for functional genomics in plants and crop improvement. The spec- ificity of Cas-directed DNA cleavage is strictly determined by a chimeric single guide RNA （sgRNA） and a short protospacer adjacent motif （PAM） in the genome （Cong et al., 2013; Zetsche et al., 2015）. The widely used SpCas9 and its variants have been shown to recognize PAM sequences in the canonical form NGG and non-canonical NGA, NAG, or NGCG in plants （Miao et al., 2013; Ma et al., 2015; Hu et al., 2016）. CRISPR/Cpfl, a new class 2 CRISPR/Cas system, was recently exploited as an alternative tool for genome editing in various organisms, including plants （Zetsche et al., 2015; Kim et al., 2017; Tang et at., 2017; Wang et al., 2017; Xu et al., 2017）. Cpfl utilizes a thymidine-rich PAM site, TTTN, and is guided by a single CRISPR RNA （crRNA） （Zetsche et al., 2015）.Dear Editor The CRISPR/Cas gene editing system offers great potential for functional genomics in plants and crop improvement. The spec- ificity of Cas-directed DNA cleavage is strictly determined by a chimeric single guide RNA （sgRNA） and a short protospacer adjacent motif （PAM） in the genome （Cong et al., 2013; Zetsche et al., 2015）. The widely used SpCas9 and its variants have been shown to recognize PAM sequences in the canonical form NGG and non-canonical NGA, NAG, or NGCG in plants （Miao et al., 2013; Ma et al., 2015; Hu et al., 2016）. CRISPR/Cpfl, a new class 2 CRISPR/Cas system, was recently exploited as an alternative tool for genome editing in various organisms, including plants （Zetsche et al., 2015; Kim et al., 2017; Tang et at., 2017; Wang et al., 2017; Xu et al., 2017）. Cpfl utilizes a thymidine-rich PAM site, TTTN, and is guided by a single CRISPR RNA （crRNA） （Zetsche et al., 2015）.

分 类 号：Q522[生物学—生物化学] TN946[电子电信—信号与信息处理]