二仙汤改善去卵巢大鼠学习记忆能力的海马蛋白质组学研究  

Hippocampus proteomics for effect of Er-xian decoction on ovariectomy induced-learning and memory disabilities in rats

作　　者：刘波[1] 刘志文[1] 王莹[1] 吴琪[1] 杨硕[1] 谢慧慧[1] LIU Bo;LIU Zhi-wen;WANG Ying;WU Qi;YANG Shuo;XIE Hui-hui(School of Pharmacy,Jiangxi University of Traditional Chinaese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学药学院

出　　处：《中国中药杂志》2018年第14期2991-2998,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81560723);江西省自然科学基金项目(20151BAB205072)

摘　　要：基于蛋白质组表达的差异性探讨二仙汤改善去卵巢大鼠学习记忆能力的作用机制。实验动物按体质量随机分为3组,即假手术组、模型组、二仙汤组,其中假手术组和模型组灌服生理盐水,二仙汤组则按生药量12 g·kg^-1灌服二仙汤,连续给药90 d后,采用Morris水迷宫考察大鼠的学习记忆能力,以Nano-LC-LTQ-Orbitrap系统检测海马蛋白,Protein Discovery软件进行蛋白质鉴定,SIEVE软件对蛋白进行相对定量分析,DIVAD数据库对差异蛋白进行生物学功能分析。结果显示,与模型组相比,二仙汤组逃避潜伏期明显缩短,穿越平台次数、平台象限的停留时间（TP）和TP与总游泳时间（T）的百分比（TP/T）均增加。与模型组相比,二仙汤组共发现216个与假手术组趋势一致的差异蛋白,主要富集于Ca^2＋信号通路,Rab3A与Rab14为functional classification富集分值最高的基因组中的关键基因。结果表明,二仙汤能提高去卵巢大鼠的学习记忆能力,Ca^2＋信号通路以及Ras-related protein Rab-3A,Ras-related protein Rab-14等相关差异蛋白质可能与二仙汤的干预效应有关。To investigate the effect of Er-xian decoction on ovariectomy induced-learning and memory disability in rats based on the differences in hippocampus proteomic expression,the female rats were ovariectomized for perimenopausal status and then randomly divided into 3 groups,namely sham group,control group and Er-xian decoction group. The rats in sham group and control group were given normal saline by gavage everyday,the rats in Er-xian decoction group were given 12 g·kg^-1 of Er-xian decoction by gavage once a day. After treatment for 90 day,all of the animals were tested by Morris water maze,and all of the samples were collected for testing. The hippocampus proteins were detected by Nano-LC-LTQ-Orbitrap system,and identified by Protein Discovery software. The intensity of differential proteins were quantitated by SIEVE software,and the biological functions of protein were analyzed by DIVAD database. The results showed that Er-xian decoction could significantly improve the abilities of learning and memory. Compared with control group,216 differential proteins with a consistent variation trend with the sham group were found in the Er-xian decoction group. And they mainly concentrated in calcium signaling pathway,Rab3 A and Rab14 were key genes with the highest functional classification enrichment score in the gene group. Er-xian decoction could significantly improve the abilities of learning and memory in perimenopausal model rats. The invention effect of Er-xian decoction may be correlated with the calcium signaling pathway and Ras-related protein Rab-3 A,and Ras-related protein Rab-14.

关 键 词：二仙汤 围绝经期 学习记忆 蛋白质组学 海马 

分 类 号：R285.5[医药卫生—中药学]