上海口岸1只进境犬携带蜱的种类鉴定与溯源分析  

Identification and traceability of an intercepted tick from imported dog at Shanghai port

作　　者：王强 何宇平[1] 吴成云[1] 王巧全[1] 王艳[1] 林颖峥 陈志飞[1] 张强[1] 邓耀华[1] 张冠楠[1] 曹宇凡 唐智芳[1] 李树清[1] WANG Qiang;HE Yu-ping;WU Cheng-yun;WANG Qiao-quan;WANG Yan;LIN Ying-zheng;CHEN Zhi-fei;ZHANG Qiang;DENG Yao-hua;ZHANG Guan-nan;CAO Yu-fan;TANG Zhi-fang;LI Shu-qing(Shanghai Entry-Exit Inspection and Quarantine Bureau,Shanghai 200135,China)

机构地区：[1]上海出入境检验检疫局,上海200135

出　　处：《中国媒介生物学及控制杂志》2018年第4期340-343,共4页Chinese Journal of Vector Biology and Control

基　　金：国家重点研发计划项目(2016YFC1202000;2016YFC1202004)~~

摘　　要：目的对上海口岸截获的德国成犬体表蜱进行种类鉴定及溯源分析。方法 2016年11月28日在上海口岸进口的69只德国警犬中的第33号警犬右前肢内侧检获蜱1只,对其进行形态学鉴定,并提取其DNA,扩增线粒体16S r RNA基因进行测序、比对,并对感染途径进行溯源分析。结果形态学鉴定该蜱符合血蜱属的形态特征,分子鉴定表明该蜱的线粒体基因序列与广泛分布于古北区和东洋区的褐黄血蜱(Haemaphysalis flava Neumann,1897)线粒体基因匹配度达98%。结论截获蜱为褐黄血蜱,为犬入境后感染。Objective To identify the unknown species of ticks carried by the dog imported from Germany to China.Methods Use morphological identification,and extract the genomic DNA from the ticks,amplify the mitochondrial 16 S r RNA sequences and sequencing.Blast in the NCBI,to identify the species of the ticks collected from Shanghai port on November 28,2016.Results Morphological analysis revealed that the tick was in accordance with the morphological characteristics of Haemaphysalis.The sequence analysis results showed that the measured gene of this tick was 98%identical to the mitochondrial gene sequence of Ha.flava Neumann,1897 which was widely distributed in Palaearctic and Oriental region.Conclusion This tick is Ha.flava,and infected the dog after entry.

关 键 词：分子鉴定 褐黄血蜱 上海口岸 

分 类 号：R384.4[医药卫生—医学寄生虫学]