东北地区蜱传斑点热群立克次体的分子流行病学研究  

Molecular epidemiological studies on spotted fever group rickettsia in ticks from Northeastern China

作　　者：王卓[1] 王建伟 于淼[1] 邢悦鹏 冯立[1] 杨义军[1] 王秀红[1] 田圃[2] 吴益民[1] WANG Zhuo;WANG Jian-wei;YU Miao;XING Yue-peng;FENG Li;YANG Yi-jun;WANG Xiu-hong;TIAN pu;WU Yi-min(l Center of Disease Prevent and Control of Shenyang Command,Shenyang 110034,Liaoning Province,China;Jilin University,College Life Science;Liaoning Institute for Food Control)

机构地区：[1]沈阳军区疾病预防控制中心,沈阳110034 [2]吉林大学生命科学学院,长春130012 [3]辽宁省食品检验检测院,沈阳110016

出　　处：《中国媒介生物学及控制杂志》2018年第4期344-347,共4页Chinese Journal of Vector Biology and Control

基　　金：国家科技重大专项(2016ZX10004203-001-009);军队应用基础研究项目(AWS11L009)~~

摘　　要：目的调查东北地区蜱感染斑点热群立克次体(SFGR)情况及其种型分布。方法 2012-2014年5-6月,采用人工布旗法在东北3省9个市(县)和黑瞎子岛不同生境采集游离蜱以及家畜(牛、羊)体表寄生吸血蜱并鉴定;采用PCR方法对蜱种进行检测,对检出的阳性样本进行测序和序列分析,并建立分子系统进化树。结果蜱的SFGR阳性率为11.43%,不同地区SFGR阳性率差异无统计学意义(χ~2=7.683,P=0.566);不同蜱种SFGR感染率差异亦无统计学意义(χ~2=6.354,P=0.174)。5份嗜群血蜱标本及1份日本血蜱标本、1份森林革蜱标本与Rickettsia heilongjiangensis 054株(AF179362.2)和HL-93株(AF179364.1)聚为一支,同源性为99.30%~100%;3份长角血蜱标本与Candidatus R.hebeiii(QHD-3.HQ651817.1、TS-1.HQ651818.1、QHD-1.HQ651815.1)聚为一支,同源性为99.83%~100%;4份森林革蜱标本与R.sp.Dn S14(AF009130.2)和吉林株R.sp.JL-02(AY093696.1)聚为一支,同源性为98.79%~100%。结论东北地区蜱类SFGR感染普遍,存在感染SFGR蜱种的多样性和SFGR基因型多样性特点,提示该地区人群应重视对蜱传斑点热的防护,同时应加强患者症状的鉴别与诊断能力。Objective To investigate the prevalence and diversity of spotted fever group rickettsia infection（SFGR）in ticks in northeastern China.Methods Flagging for free-living ticks and off-host（cattle and sheep）collection for parasitic ticks were conducted from 9 counties and Heixiazi Island from Northeastern China,during May to June,2012-2014.Conventional PCR was carried out using universal SFGR primers targeting omp A genes to screen for their infection with SFG rickettsia organisms in ticks collected.The positive products were sequenced,and molecular phylogenetic tree was established.Results The presence of SFGR was 32 of 280 pools（1 224 ticks）tested,with overall positive rate 11.43%.There were no significant differences in different regions（χ^2=7.683,P=0.566）and in different tick species（χ^2=6.354,P=0.174）for their infection with SFGR rickettsia.The phylogenetic analysis based on nucleotide sequence showed that 14 SFGR DNA sequences belonged to 3 SFGR species：5 samples from Dermacentor silvarum,1 from each of Haemaphysalis concinna and H.japonica were clustered together with Rickettsia heilongjiangensis 054（AF179362.2） and HL-93（AF179364.1）,showing 99.30%-100% identity;3 samples from H.longicornis were clustered with Candidatus R.hebeiii（QHD-3.HQ651817.1,TS-1.HQ651818.1,QHD-1.HQ651815.1）with 99.83%-100% homogeny;4 samples from D.silvarum were clustered together with R.sp.Dn S14（AF009130.2）,and Jilin Strain R.sp.JL-02（AY093696.1）with 98.79%-100% identity.Conclusion The prevalence of SFGR in ticks from Northeastern China was high.There was high diversity in SFG rickettsia species and tick species in the areas surveyed.

关 键 词：蜱 斑点热群立克次体 聚合酶链式反应 序列分析 

分 类 号：R384.4[医药卫生—医学寄生虫学] R376[医药卫生—基础医学]