EAAT_2调节异氟烷长时程处理对星形胶质细胞的损伤作用  

EAAT_2 mediates prolonged isoflurane treatment-induced cytotoxicity in astrocytes

作　　者：王静怡 郭海云 樊泽 Wang Jingyi;Guo Haiyun;Fan Ze(Department of Anesthesiology and Perioperative Medicine,Xijing Hospital,Fourth Military Medical University,Xi＇an 710032,China)

机构地区：[1]第四军医大学西京医院麻醉与围术期医学科,西安710032

出　　处：《神经解剖学杂志》2018年第4期420-426,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81420108013);西京助推基金(xjzt15m02)

摘　　要：目的:验证吸入麻醉剂异氟烷长时程处理对星形胶质细胞的毒性作用并探讨其可能的分子机制。方法:将原代星形胶质细胞在异氟烷中进行长时程暴露,通过MTT检测星形胶质细胞的活性,利用Western Blot和qRT-PCR检测EAAT_2的表达。利用慢病毒构建EAAT_2体外过表达和沉默模型并探索干预后对异氟烷诱导星形胶质细胞毒性作用的影响。结果:(1)异氟烷长时程处理损害星形胶质细胞的活性,并呈剂量(P<0.01)和时间(P<0.001)依赖性;(2)异氟烷长时程处理引起EAAT_2表达增加,且增加程度与异氟烷剂量(P<0.05)和暴露时间(P<0.001)呈正相关;(3)下调星形胶质细胞EAAT_2的表达可以减轻异氟烷长时程处理对星形胶质细胞的损伤(P<0.05)。结论:EAAT_2可调节异氟烷长时程处理对星形胶质细胞的损伤作用。Objective：To verify isoflurane treatment-induced cytotoxicity in astrocytes,and investigate the possible molecular mechanism for this process.Methods：Primary astrocytes were long-term exposed to isoflurane.The activity of astrocytes was detected by MTT assay.The expression of EAAT2 was detected by Western Blot and q RTPCR.Using lentiviruses to construct the EAAT2 over-expression and knockdown models,and explore the role of EAAT2 in isoflurane treatment-induced cultured astrocytic cytotoxicity.Results：（1） Prolonged exposure to isoflurane decreased primary-astrocytic viability in a dose-dependent（P〈0.01） and time-dependent（P〈0.001） manner;（2）Prolonged exposure to isoflurane increased the expression of EAAT2 in a dose-dependent（P〈0.05） and time-dependent（P〈0.001） manner;（3） EAAT2 knockdown promoted the activity of astrocytes（P〈0.05） following isoflurane exposure.Conclusion：EAAT2 can mediate prolonged isoflurane treatment-induced cytotoxicity in primary astrocytes.

关 键 词：EAAT2 异氟烷 长时程麻醉 星形胶质细胞 

分 类 号：R614[医药卫生—麻醉学]