EP2受体抑制剂AH6809对单纯性脑震荡大鼠皮质中肿瘤坏死因子表达的调控  

The effect of EP2 receptor inhibitor AH 6809 on the expression of TNF-α in cortex of rats after pure cerebral concussion

作　　者：蒋鸿雁 吴海鹰[2] 吴德野 王华伟[4] 李恒希 于建云 李坪[1] Jiang Hongyan;Wu Haiying;Wu Deye;Wang Huawei;Li Hengxi;Yu Jianyun;Li Ping(Kunming Medical University ： 1.Depaament of Anatomy and Histology ＆ Embryology;First Affiliated Hospital of Kunming Medical University,Department of Emergency;Department of Forensic Medicine and Lab of Kunming Medical University,Department of Emergency;Department of Forensic Medicine and Lab of Trauma Brain Injury Research;First Affiliated Hospital of Kunming Medical university,Department of Reproduction and Genetics,Kunming 650500;Department of Human Anatomy and Histology ＆ Embryology,Qilu Medical University,Zibo 255213,China)

机构地区：[1]昆明医科大学人体解剖学与组织胚胎学系,昆明650500 [2]昆明医科大学第一附属医院急诊医学科,昆明650500 [3]齐鲁医药学院人体解剖学与组织胚胎学系,山东255213 [4]昆明医科大学第一附属医院生殖遗传科,昆明650500 [5]昆明医科大学法医学院及脑损伤研究室,昆明650500

出　　处：《神经解剖学杂志》2018年第4期441-446,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(31100769);云南省教育厅科学研究基金资助项目(2017z DX164);云南省哲学社会科学规划项目(YB2016031)

摘　　要：目的:观察单纯性脑震荡(pure cerebral concussion,PCC)后使用AH6809干预大鼠前额叶皮质(prefrontal cortex,PC)、颞叶皮质(temporal cortex,TC)和梨状皮质(piriform cortex,Pir)肿瘤坏死因子(tumor necrosis factor-α,TNF-α)的表达及其变化规律,探索AH6809对脑损伤后TNF-α的调控作用。方法:在清醒状态下,采用"金属单摆式闭合脑损伤打击装置"复制PCC模型,分别于损伤后即刻和12 h腹腔注射AH6809(EP2受体抑制剂)进行干预,随机分为1、2、3和7 d的PCC损伤组(n=5),另设生理盐水(NS)对照组(n=5)。通过免疫组化和Western Blot法,观察和比较AH6809组和NS组大鼠PC、TC和Pir脑区TNF-α的表达变化。结果:AH6809干预组的TNF-α阳性表达在各个时间点较NS组明显减弱,差异具有显著性(P<0.05)。结论:AH6809对PCC大鼠致伤后TNF-α的高表达具有一定抑制作用,一定程度上减轻炎症反应。Objective：To observe the expression of tumor necrosis factor-α（TNF-α） in the prefrontal cortex（PC）,temporal cortex（TC） and piriform cortex（Pir） of rats subjected by pure cerebral concussion（PCC） after the intervention of AH6809 and explore the regulation of AH6809 on TNF-α after brain injury.Methods：The PCC models of rats were duplicated by ＂metallic pendulum-striker closed brain injury device＂under awake condition.AH6809（an EP2 receptor inhibitor） was administered intraperitoneally immediately and at 12 hours after PCC and the rats were randomly divided into groups（n = 5） at 1,2,3 and 7 d after injury.The corresponding control rats received the injection of normal saline（NS）.The expression of TNF-α in PC,TC and Pir areas in rats was evaluated by immunohistochemistry and Western Blot after PCC followed by AH6809 and NS treatment.Results：The expression of TNF-α in PC,TC and Pir in AH6809 group was weaker than that in NS groups at various time points following PCC（P〈0.05）.Conclusion：AH6809 showed a certain inhibitory effect on the high expression of TNF-α after PCC in rats,which can reduce the inflammatory reaction to some extent.

关 键 词：单纯性脑震荡 大脑皮质 TNF-α AH6809 大鼠 

分 类 号：R651.15[医药卫生—外科学]