铝镁钙复合作用对杉木幼苗叶片丙二醛(MDA)含量及抗氧化酶活性的影响  

Effects of Combined Effects of Aluminum,Magnesium and Calcium on MDA Content and Antioxidant Enzyme Activities in Leaves of Chinese Fir Seedlings

作　　者：严绍裕[1] YAN Shao-yu(Fujian Forestry Vocational Technical College,Nanping,Fujian 35300)

机构地区：[1]福建林业职业技术学院,福建南平353000

出　　处：《安徽农业科学》2018年第25期92-95,100,共5页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究不同铝、镁、钙复合处理对杉木幼苗叶片丙二醛(MDA)含量及抗氧化酶活性的影响。[方法]以1年生不同耐铝型杉木实生苗为研究对象,采用正交试验设计,设置铝、镁、钙复合共计16个处理,研究铝、镁、钙复合处理对不同耐铝型杉木幼苗叶片MDA含量和抗氧化酶活性的影响。[结果]耐铝型杉木幼苗叶片MDA含量与敏感型相比其含量较低;耐铝型杉木幼苗叶片超氧化物歧化酶(SOD)活性受铝离子的影响较快,而敏感型受镁离子影响较快;耐铝型杉木幼苗叶片过氧化物酶(POD)活性受钙离子的影响最快,且随着时间的增加其影响作用逐渐增大,而敏感型杉木幼苗叶片POD活性受铝离子的影响最快,且影响作用逐渐增大;耐铝型杉木幼苗叶片过氧化氢酶(CAT)活性受镁离子的影响最快,钙离子则对敏感型的影响最快,随着胁迫处理时间的延长,不同耐铝型杉木幼苗叶片CAT活性的主要影响因素均为铝离子。[结论]该研究可为杉木铝毒害作用的研究提供一定的理论基础。[ Objective]To study the effects of combined effects of aluminum,magnesium and calcium on MDA content and antioxidant enzyme activities in leaves of Chinese fir seedlings. [Method] The effects of aluminum,magnesium and calcium on the MDA content and antioxidant enzyme activity in the leaves were studied by orthogonal experiment design with 16 treatments of alwith 1 year old Chinese fir seedlings. [ Result] The content of MDA in leaves of aluminum tolerant Chinese fir seedlings was lower than that ofsensitive ones. The activity of SOD in leaves of aluminum tolerant Chinese fir seedlings was afected by aluminum ions faster,while sensitive type was more afected by magnesium ions. The effect of calcium ion on the POD activity in leaves of Chinese fir seedlings was the most impor-tant ,and with the increase of time , the effect of POD in leaves of sensitive Chinese fir seedlings was the fastest , and the effect increased grad-ually. The effect of magnesium ion on the CAT activity in leaves of aluminum resistant Chinese fir seeeiings was the fastest,and the calcium ion had the fastest effect on the sensitive type. With the prolonged stress treatment time,the main factors afecting the CAT activity in leaves of different aluminum resistant Chinese fir seedlings were aluminum ions. [ Conclusion ] The study provides theoretical basis of the study of alumi-num toxicity of Chinese fir.

关 键 词：杉木幼苗 铝毒 铝镁钙复合作用 丙二醛 抗氧化酶活性 

分 类 号：S718.43[农业科学—林学]