眼镜蛇毒因子抑制补体激活对脓毒症大鼠血清细胞因子的影响  

Effect of Cobra Venom Factor in Inhibiting Complement Activation on Serum Cytokines in Septic Rats

作　　者：魏乔[1] 盛雅倩[1] 茹松伟[2] 康福新 李国平[4] 张民伟[1] WEI Qiao;SHENG Yaqian;RU Songwei(The First Affiliated Hospital of Xiamen University,Xiamen361003,China)

机构地区：[1]厦门大学附属第一医院,福建厦门361003 [2]航天中心医院 [3]海南医学院第二附属医院 [4]南昌大学第二附属医院

出　　处：《中国医学创新》2018年第23期31-35,共5页Medical Innovation of China

基　　金：江西省自然科学基金资助项目(0440083)

摘　　要：目的:观察眼镜蛇毒因子(cobra venom factor,CVF)对脓毒症大鼠外周血中肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)和白介素-10(IL-10)的影响。方法:选取清洁级雄性SD大鼠144只,按照随机数字表法将大鼠分为A组(假手术组)、B组(脓毒症组)、C组(CVF预处理组),各48只;各组按照标本采集点的不同又分为术后2、4、8、12、16、24 h六个时间点亚组,各8只。B、C组采用盲肠结扎穿孔术(CLP)制作脓毒症大鼠模型;C组大鼠在CLP术前静脉注射CVF,而A、B组静脉注射等量0.9%氯化钠溶液。观察术后大鼠一般情况及腹腔情况,比较各组术后不同时间点的TNF-α、IL-6和IL-10水平变化。结果:C组大鼠脓毒症反应较B组明显减轻;除术后8 h外,C组术后其他时间点TNF-α水平均低于B组(P<0.05),其中B组术后2 h时TNF-α迅速升高达峰然后下降,但是术后12 h再次升高出现第二个峰值,C组术后4 h较2 h迅速下降,术后8 h再次升高出现第二个峰值;除术后16 h外,C组术后其他时间IL-6水平均低于B组(P<0.01);除术后2、24 h外,C组血清IL-10水平均高于B组(P<0.05),而且术后8、16 h两次达到峰值,呈双峰变化。结论:CVF可以有效维持促炎细胞因子(TNF-α、IL-6)和抗炎细胞因子(IL-10)的平衡并明显抑制脓毒症大鼠的炎症反应,同时TNF-α及IL-10的"双峰现象"也为免疫平衡理论及PICS提供了理论依据。Objective：To observe the effect of cobra venom factor（CVF） on TNF-α,IL-6 and IL-10 in peripheral blood of septic rats.Method：A total of 144 male SD rats with clean grade were selected.According to the random number table method,rats were divided into group A（sham operation group）,group B（sepsis group） and group C（CVF pretreatment group）,48 cases in each group.According to the different collection points,each group was divided into six subgroups（after operation 2,4,8,12,16,24 h）,8 cases in each subgroup.The sepsis rats were induced by cecal ligation and puncture（CLP） in group B and C,and the rats of group C received intravenous injection of CVF before CLP,while those of group A and B received equal amount of normal saline intravenously.The general condition and abdominal cavity condition of rats after operation were observed,and the levels of TNF-α,IL-6 and IL-10 at different time points after operation in each group were compared. Result：The manifestations of sepsis in group C were less severe than those of group B.Except for 8 h after operation,the levels of TNF-αin group C were lower than those of group B（P〈0.05）.The levels of TNF-α in group B increased rapidly to peak at 2 h,and then decreased,but increased again to the second peak at 12 h,and the levels of TNF-α in group C decreased rapidly at 4 h compared with 2 h and increased again to the second peak at 8 h.Except for 16 h after operation,the levels of IL-6 in group C were lower than those of group B（P〈0.01）.Except for 2 and 24 h after operation,the levels of serum IL-10 in group C were higher than those of group B（P〈0.05）,and peaked at 8 h and 16 h after operation,which showed bimodal changes.Conclusion：CVF can effectively maintain the balance of pro-inflammatory cytokines（TNF-α,IL-6） and anti-inflammatory cytokine（IL-10）,and obviously inhibit the inflammatory response of sepsis rats.The bimodal phenomenon of TNF-α and IL-10 also provides a theoretical basis for the theory of immune balance

关 键 词：脓毒症 补体 眼镜蛇毒因子 细胞因子 

分 类 号：R459.7[医药卫生—急诊医学]