迟缓爱德华氏菌可视化核酸试纸条检测方法的研究  

Research on a Visual Nucleic Acid Dipstick Assay for Rapid Detection of Edwardsiella tarda

作　　者：房敬真 房保海[2] 徐守振[1] FANG Jingzhen;FANG Baohai;XU Shouzhen(College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China;Inspection and Quarantine Technology Center of Shandong Entry-Exit Inspection and Quarantine Bureau,Qingdao 266002,China)

机构地区：[1]青岛农业大学动物医学院,山东青岛266109 [2]山东出入境检验检疫局检验检疫技术中心,山东青岛266002

出　　处：《青岛农业大学学报（自然科学版）》2018年第3期220-223,共4页Journal of Qingdao Agricultural University(Natural Science)

基　　金：国家质检总局科技项目(2012IK018);山东检验检疫局科技项目(SK201403);山东省自然科学基金面上项目(ZR2016CM08)

摘　　要：为建立迟缓爱德华氏菌的快速检测方法,本研究根据迟缓爱德华氏菌溶血相关基因eha设计保守引物,建立迟缓爱德华氏菌快速、可视化核酸试纸条检测技术。试验结果显示,该技术快速、灵敏、具有良好的特异性,灵敏度可达到30CFU/mL,比琼脂糖凝胶成像检测技术灵敏度要高近10倍。迟缓爱德华氏菌可视化核酸试纸条检测方法的成功建立对水产养殖过程中迟缓爱德华氏菌病防治具有重要的意义。To establish the rapid detection method for Edwardsiella tarda,apair of conservative primers was designed and a visual nucleic acid dipstick assay for rapid detection of E.tarda was established according to hemolytic gene eha.The results showed that this assay method was rapid,sensitive and highly specific.Its sensitivity reached 30 CFU/mL and was nearly 10 times than that of agarose gel imaging technology.This technique is of great significance for the prevention and treatment of edwardsiellasis in aquaculture.

关 键 词：迟缓爱德华氏菌 核酸试纸条 可视化检测 

分 类 号：S941.42[农业科学—水产养殖]