GLI-1信号途径参与低氧诱导的宫颈癌Hela细胞侵袭的作用机制研究  

Mechanism of GLI-1 in aggressive behavior of cervical cancer Hela cell induced by hypoxia

作　　者：陈静 邵勇[2] 陈诚 袁晓剑 CHEN Jing;SHAO Yong;CHEN Cheng;YUAN Xiao-jian(Department of Gynaecology and Obstetrics,People s Hospital of Jiulongpo District in Chongqing,Chongqing 400050,China;Department of Gynaecology and Obstetrics,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400042,China;Department of Gynaecology and Obstetrics,the First Affiliated Hospital of Army Medical University,Chongqing 400038,China;Department of Surgery,Xipeng Hospitals of Jiulongpo District in Chongqing,Chongqing 401326,China)

机构地区：[1]重庆市九龙坡区人民医院妇产科,重庆400050 [2]重庆医科大学附属第一医院妇产科,重庆400042 [3]陆军军医大学第一附属医院妇产科,重庆400038 [4]重庆市九龙坡区西彭镇卫生院外科,重庆401326

出　　处：《局解手术学杂志》2018年第8期545-549,共5页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金(30972894)

摘　　要：目的探讨GLI-1在低氧微环境介导的宫颈癌Hela细胞侵袭中的作用及其机制。方法分别在低氧(1%O2)和常氧(21%O2)环境下体外培养宫颈癌Hela细胞,采用Transwell侵袭试验检测各组细胞侵袭能力的变化;同时采用RT-PCR和Western blot法检测细胞中GLI-1 mRNA和蛋白表达。结果低氧微环境能够增强宫颈癌Hela细胞的侵袭能力,同时在低氧环境下,GLI-1基因能够被激活。阻断GLI-1信号途径后,Hela细胞侵袭能力显著降低,且伴随着GLI-1基因的表达下调。结论在低氧条件下,Hela细胞侵袭能力增强,其机制可能是通过激活GLI-1的表达增强来实现的。Objective To investigate the mechanism of GLI-1 in aggressive behavior of cervical cancer Hela cell induced by hypoxiawhether hypoxia. Methods For the current study,Hela cells were incubated in normoxia and hypoxia conditions respectively.The mRNA and protein expression levels of GLI-1 was monitored by RT-PCR and Western blot,cell invasion abilities were detected by Transwell invasion assay again. Results The results demonstrated that hypoxia micro-environment was able to up-regulating the expression levels of GLI-1 and enhance the invasion potentiality of cervical cancer Hela cells.Furthermore,blocking of GLI-1 by using specific siRNA could significantly inhibit the invasion ability of Hela cells in hypoxia,with down-regulating the expression of GLI-1 gene. Conclusion The invasion ability of cervical cancer Hela cells was enhanced by upregulating the expression of GLI-1 in hypoxia.

关 键 词：宫颈癌 低氧微环境 GLI-1 侵袭 

分 类 号：R711.74[医药卫生—妇产科学]