光固定明胶修饰的聚醚醚酮(PEEK)表面促进细胞增殖及成骨性研究  

Surface decorated polyetheretherketone(PEEK) with photoreactive gelatin enhancing cell proliferation and differentiation

作　　者：万腾[1] 张郡[1] 田海清[1] 袁天阳[1] 刘钦毅[1] Wan Teng;Zhang Jun;Tian Haiqing(Deparment of Orthopedics,Second Affiliated Hospital of Jilin University,Changchun Jilin,130000,China)

机构地区：[1]吉林大学第二医院骨科,吉林长春130000

出　　处：《生物骨科材料与临床研究》2018年第4期9-11,15,共4页Orthopaedic Biomechanics Materials and Clinical Study

摘　　要：目的聚醚醚酮(PEEK)具有与骨骼相似的弹性模量,但PEEK作为骨修复材料的应用受到其表面生物惰性及缺乏促成骨性的限制。为了解决这个问题,我们通过表面修饰光固定明胶增强PEEK生物活性。方法我科研团队合成的光固定明胶可通过紫外照射粘附于多种材料表面,并可增强材料的生物相容性。进行SEM、静态水接触角、细胞增殖、细胞形态、碱性磷酸酶分化等表征学及细胞学系统研究。结果研究表明光固定明胶可固定于PEEK表面,改变了PEEK等高分子材料的表面性质。在细胞学研究中,光固定明胶改性后相对于普通PEEK,成骨细胞增殖、伸展、基质分泌及分化能力明显增强。结论通过光固定明胶对PEEK表面进行改性,可明显增强其生物活性,是一种有潜力的骨科内植入物及医疗器械材料。Objective Polyetheretherketone（PEEK） has an elastic modulus similar to the bone; however, its use as a material for bone repair is limited by bio-inert surface and poor osteogenesis-inducing capacity. To address this issue, the PEEK surface was docerated with photoreactive gelatin for enhancing biocompatibility. Methods A photoreactive gelatin（Az-Gel）, which could be immobilized on various matrices by UV-irradiated, was synthesized by our group to improve the matrices biocompatibility. The surface characteristics and in vitro cellular behavior are systematically investigated by scanning electron microscopy, static contact angles, cell proliferation assay, alkamine phosphatase activities and cellular morphology. Results Our study demonstrated that Az-Gel was immobilized on the surfaces of PEEK and could change the surface properties of polymer substrates. In vitro experiment, MC3T3-E1 cells showed increased proliferation, cell spreading, cellular matrix secretion and osteogenic differentiation capacity on Az-Gel-modified as compared to unmodified PEEK. Conclusion PEEK that surface-modified with photoreactive gelatin can enhance biocompatibility and is a potential material for use in orthopedic implants and medical devices.

关 键 词：聚醚醚酮(PEEK) 光固定明胶 表面改性 

分 类 号：R318.01[医药卫生—生物医学工程]