雌马酚对糖尿病大鼠心肌内质网应激和细胞凋亡的影响  

Effect of equol on myocardial endoplasmic reticulum stress and apoptosis in diabetic cardiomyopathy rats

作　　者：白跳 迟丽屹[2] 高宇勤 胡小菁 BAI Tiao;CHI Liyi;GAO Yuqin;HU Xiaojing(First Department of Geriatrics,Ninth Hospital of Xi＇an Xi＇ an 710054,China;Department of Neurology,451th Hospital of PLA;Department of Cardiovascular Medicine,Ninth Hospital of Xi＇an)

机构地区：[1]西安市第九医院老年病一科,西安710054 [2]解放军第四五一医院神经内科 [3]西安市第九医院心血管内科

出　　处：《山西医科大学学报》2018年第8期886-889,共4页Journal of Shanxi Medical University

基　　金：国家自然科学基金面上项目(81673787)

摘　　要：目的探讨雌马酚对糖尿病心肌病大鼠心肌内质网应激和细胞凋亡的影响。方法 24只8周龄雄性Wistar大鼠随机分为对照组,糖尿病组和雌马酚组,每组8只。对照组喂以普通饲料,糖尿病组和雌马酚组大鼠喂以高糖高脂饲料。饲养第56天(第8周),糖尿病组及雌马酚组大鼠分别腹腔注射链尿佐菌素45 mg/kg一次,制备糖尿病模型。此后,雌马酚组大鼠改为食用含0.1%雌马酚的高糖高脂饲料,糖尿病组和对照组大鼠继续饲喂原方案饲料。链尿佐菌素注射后第12周,测定体质量、血清甘油三酯和总胆固醇水平及空腹血糖,用Western blot蛋白检测法测定心肌GRP78、CHOP、ATF-6、活化caspase-3、Bax及Bcl-2表达水平。结果与对照组比较,糖尿病组大鼠体质量显著下降,血清总胆固醇、空腹血糖水平明显升高(P<0.05),血浆LDH、CK-MB、AST水平增高(P<0.05),心肌内质网应激相关指标GRP78、CHOP、ATF-6显著升高(P<0.05),且活化caspase-3蛋白和促凋亡蛋白Bax显著增高,抗凋亡蛋白Bcl-2减少(P<0.05)。与糖尿病组比较,雌马酚组大鼠体质量增加,血清总胆固醇、空腹血糖水平明显降低(P<0.05),血浆心肌酶LDH、CK-MB、AST水平减少(P<0.05),心肌内质网应激相关指标GRP78、CHOP、ATF-6显著减少(P<0.05),且活化caspase-3蛋白和促凋亡蛋白Bax表达减少,抗凋亡蛋白Bcl-2表达增多(P<0.05)。结论雌马酚可减轻糖尿病大鼠心肌内质网应激及细胞凋亡。Objective To explore the effect of equol on myocardial endoplasmic reticulum stress and apoptosis in diabetic cardiomyopathy rats.Methods Twenty-four male Wistar rats（8 weeks old） were randomly divided into three groups（n = 8 in each group） ：control group,diabetic group,and equol group.The rats in control group were fed with normal diet,while the rats in diabetic group and equol group were fed with high-sugar and high-fat diets.After 8-week feeding,the rats in diabetic group and equol group were intraperitoneally injected with 60 mg/kg streptozotocin to build up a diabetic model.Then,the rats in equol group were given a high-sugar and high-fat diet containing 0.1% equol,while the rats in diabetic group and control group were still fed continuously according to the original protocol.Twelve weeks after the injection of streptozotocin,body weight,triglyceride,total cholesterol and plasma fasting glucose were measured.The expression levels of GRP78,CHOP,ATF-6,activated caspase-3,Bax and Bcl-2 in the myocardium were measured by Western blot.Results Compared with control group,the weight of rats significantly decreased in diabetic group,the levels of total cholesterol and the fasting blood glucose increased significantly（P 0.05）,the plasma levels of myocardial enzymes LDH,CKMB,AST increased（P 〈0.05）,the endoplasmic reticulum stress-related markers GRP78,CHOP,ATF-6 were significantly increased（P 〈0.05）,the activated caspase-3 protein and pro-apoptotic protein Bax were significantly increased,and the anti-apoptotic protein Bcl-2 was decreased（P〈0.05）.Compared with diabetic group,the weight of rats was increased in equol group,while the total cholesterol and fasting blood glucose levels were significantly decreased（P 〈0.05）,and the plasma levels of myocardial enzymes LDH,CKMB,and AST were decreased（P 0.05）.Compared with diabetic group,the protein expression of GRP78,CHOP and ATF-6 were significantly decreased in equol group（P 〈0.05）,the protein expression of activated caspas

关 键 词：雌马酚 糖尿病 糖尿病心肌病 内质网应激 细胞凋亡 

分 类 号：R541.8[医药卫生—心血管疾病]