LPA和COX-2在鼠肺纤维化模型中的表达及意义  

EXPRESSION AND SIGNIFICANCE OF LPA AND COX-2 IN RAT MODEL OF PULMONARY FIBROSIS

作　　者：顾岩[1] 张松[1] 王小雨 张敏[1] 付秀华[1] GU Yan;ZHANG Song;WANG Xiao-yu(The Affiliated Hospital,Inner Mongolia Medical University,Hohhot 010050 China)

机构地区：[1]内蒙古医科大学附属医院呼吸与危重症医学科,内蒙古呼和浩特010050 [2]内蒙古自治区人民医院

出　　处：《内蒙古医科大学学报》2018年第5期456-460,共5页Journal of Inner Mongolia Medical University

基　　金：内蒙古医科大学科技百万项目(YKD2015KJBW018);内蒙古医科大学附属医院重大项目(NYFYZD2014001)

摘　　要：目的:探讨LPA-COX-2在小鼠肺纤维化发生发展过程中表达及作用。方法:80只小鼠随机分为博来霉素组(BLM组)及生理盐水组(NS组),采用ELISA检测血清中溶血磷脂酸(LPA)及环氧合酶-2(COX-2)的表达;免疫组化法检测肺组织TGF-β1、EGFR、PGE2及COX-2的表达。结果:TGF-β1、EGFR、PGE2及COX-2在NS组肺脏中均有表达,但表达较弱;在BLM组的表达强于NS组。NS组血清中LPA少量表达,BLM组血清中LPA呈高表达,有显著性差异(P<0.01)。NS组血清中COX-2少量表达,BLM组小鼠14d时血清中COX-2表达量明显增加(P<0.01),随着肺纤维化的加重,小鼠血清中的COX-2表达水平逐渐降低,但较正常对照组仍有明显表达(P<0.01)。结论:LPA、COX-2、PGE2、TGF-β1和EGFR均在BLM组高表达;LPA可能通过TGF-β1途径及EGFR途径共同调节COX-2及PGE2表达。Objective：To study the expression of LPA and COX-2 in the occurrence and develop- ment of pulmonary fibrosis in rats. Methods： 80 rats were randomly divided into bleomyein group （BLM group） and saline group （NS group） , using the ELISA tests as the expression of LPA and COX -2 in serum; immuno-histoehemieal detection of lung tissue TGF- beta 1, EGFR, of PGE2 and COX -2. Results：TGF-β1, EGFR, PGE2 and COX-2 in the lungs of NS group were expressed, but the expression is weak; the expression of BLM group was better than the NS group.The LPA in serum of NS group was weakly expressed, while it was high expressed in BLM group, there was a significant difference （P〈0.01）. COX-2 in the serum of NS group was weakly expressed, while the expression of COX-2 in serum of BLM group was significant（P〈0.01 ） when mice on 14th day of the experiment. With the aggravation of pulmonary fibrosis, the expression level of serum COX-2 decreased gradually, but compared with the normal control group, the expression was still a significant difference （P〈 0. 01）.Conclusion： LPA, COX-2, PGE2, TGF-β1 and EGFR were highly expressed in the BLM group;LPA may regulate the expression of COX-2 and PGE2 by TGF-β1 pathway and EGFR pathway. In the early stage, the expression of COX-2-PGE2 may be up-regulated mainly by LPA-EGFR, and then participate in the inflammatory response of cells. In the later stage, the expression of COX-2 -PGE2 may be down regulated by LPA-TGF- beta 1, and then participate in the formation of pulmonary fibrosis.

关 键 词：肺纤维化 溶血磷脂酸 环氧合酶-2 博来霉素 

分 类 号：R563.1[医药卫生—呼吸系统]