三七总皂苷与淫羊藿总黄酮联合对自然衰老大鼠睾丸生殖功能衰退的影响  

Effects of Panax Notoginseng Saponins Combined with Total Flavonoids from Epimedium on Decline in Testicular Reproductive Function of Natural Aging Rats

作　　者：杜丽娜 王超 王芳 韩泽宇 袁丁[2] 张长城[1] 赵海霞[1] DU Li-na;WANG Chao;WANG Fang;HAN Ze-yu;YUAN Ding;ZHANG Chang-cheng;ZHAO Hai-xia(Medical College of China Three Gorge University,Yichang 443002,China;Renhe Hospital of China Three Gorge University,Yichang 443002,China)

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]三峡大学仁和医院,湖北宜昌443002

出　　处：《中国中医药信息杂志》2018年第9期31-34,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81503334;81573931;81373881;81774316)

摘　　要：目的观察三七总皂苷(PNS)与淫羊藿总黄酮(TFE)联合对自然衰老大鼠睾丸生殖功能衰退的影响,探讨其作用机制。方法将30只18月龄SD大鼠随机分为自然衰老组和PNS+TFE低、高剂量组,每组10只。另取10只2月龄大鼠为青年对照组。PNS+TFE低、高剂量组大鼠分别给予10 mg/kg PNS、TFE和20 mg/kg PNS、TFE灌胃,自然衰老组和青年对照组大鼠给予生理盐水灌胃。每周连续给药6 d,共4个月。末次给药后24 h,大鼠称重后处死,取睾丸组织,称取质量,计算睾丸指数。HE染色观察睾丸组织形态,TUNEL法检测大鼠生殖细胞凋亡,Western blot检测睾丸组织抗凋亡蛋白Bcl-2、促凋亡蛋白Bax和DNA损伤蛋白γ-H2AX的表达。结果与自然衰老组比较,PNS+TFE低、高剂量组衰老大鼠睾丸质量和睾丸指数均明显升高,生精小管组织形态明显改善,睾丸生精细胞凋亡数量明显减少,睾丸组织Bcl-2蛋白表达显著上调,Bax和γ-H2AX蛋白表达显著下调,Bax/Bcl-2比值降低。结论 PNS与TFE联合给药可延缓自然衰老大鼠生殖功能衰退,其机制可能与抑制睾丸生殖细胞的凋亡和DNA损伤有关。Objective To investigate the effects of panax notoginseng saponins（PNS） combined with total flavonoids from epimedium（TFE） on testicular dysfunction in natural aging rats; To discuss its mechanism of action. Methods Thirty 18-month old male SD rats were randomly divided into natural aging group, PNS combined with TFE low and high dose groups, with 10 rats in each group. Another 10 2-month old rats were set as young control group. PNS combined with TFE low and high dose groups were given gastric gavage of 10 mg/kg PNS combined with 10 mg/kg TFE, and 20 mg/kg PNS combined with 20 mg/kg TFE, respectively. Rats in young control group and natural aging group were given saline for 6 d each week, lasting for 4 months. Then, rats were sacrificed, and the testes were obtained to calculate the testicular weight and the testicular index. The testicular tissue morphology was observed by using HE staining. Testicular germ cell apoptosis was detected by using TUNEL method. The levels of Bcl-2, Bax and γ-H2 AX protein expression in testicular tissue were detected by Western blot. Results Compared with natural aging group, low and high dose of PNS combined with TFE significantly elevated the testicular weight and testicular index, improved the histological changes of testicular seminiferous tubule, significantly reduced number of apoptosis of spermatogenic cells in the testis, upregulated the expression of Bcl-2 protein in the testis, downregulated the expression of Bax and γ-H2 AX protein, and decreased the ratio of Bax/Bcl-2. Conclusion PNS combined with TFE can improve testicular dysfunction in natural aging rats by inhibiting apoptosis and DNA damage of germ cells.

关 键 词：三七总皂苷 淫羊藿总黄酮 衰老 凋亡 DNA损伤 大鼠 

分 类 号：R285.5[医药卫生—中药学]