补肾活血针刺法对SAMP8小鼠学习记忆能力及海马内源性神经干细胞增殖的影响  

Effects of Bushen Huoxue Acupuncture Method on Learning and Memory Abilities and Endogenous Neural Stem Cells Proliferation in Hippocampus of SAMP8 Mice

作　　者：萨依腊西.杰恩斯努尔 肖岚[1] 龙见汝 朱宏[1] 李广诚[1] 薛俐[1] 张婷[1] 任丹 李雅悦 梁梅亭 Sayrash Jiengisnur;XIAO Lan;LONG Jian-ru;ZHU Hong;LI Guang-cheng;XUE Li;ZHANG Ting;REN Dan;LI Ya-yue;LIANG Mei-ting(The Th ird Xiangya Hospital of Central South University,Changsha 410003,China)

机构地区：[1]中南大学湘雅三医院,湖南长沙410003

出　　处：《中国中医药信息杂志》2018年第9期35-38,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：湖南省科技厅计划项目(S2013F1022)

摘　　要：目的观察补肾活血针刺法对阿尔茨海默病(AD)模型SAMP8小鼠行为学及海马内源性神经干细胞增殖的影响。方法将18只7月龄雄性SAMP8小鼠作为AD模型,随机分为针刺组、非穴组和模型组,另取同月龄雄性SAMR1小鼠6只作为正常组。针刺组小鼠选取"肾俞""百会""血海""膈俞"进行针刺干预,非穴组小鼠取双侧肋下固定非穴点进行针刺,模型组和正常组小鼠予相同程度捉抓刺激,连续干预8周。采用水迷宫实验检测小鼠学习记忆能力,免疫组化检测海马Brd U阳性细胞数。结果与正常组比较,模型组小鼠逃避潜伏期明显延长(P<0.05),停留在原平台象限时间明显缩短(P<0.05),Brd U阳性细胞数明显减少(P<0.05);与模型组、非穴组比较,针刺组小鼠逃避潜伏期明显缩短(P<0.05),停留在原平台象限时间明显延长(P<0.05),Brd U阳性细胞数明显增加(P<0.05)。结论补肾活血针刺法可能通过诱导海马内源性神经干细胞的增殖,改善AD模型SAMP8小鼠的学习记忆能力。Objective To investigate the effects of Bushen Huoxue acupuncture method on the behavioral changes and the proliferation of endogenous neural stem cells（NSCs） in hippocampus of Alzheimer disease（AD） model mice. Methods 18 male SAMP8 mice, seven months old, were randomly divided into acupuncture group, non-acupoint control group, and model control group. And another age-matched 6 male SAMR1 mice were prepared as normal control group. Mice in acupuncture group were intervened by acupuncture method in the acupoints of ＂Shenshu＂, ＂Baihui＂, ＂Xuehai＂, and ＂Geshu＂. Mice in non-acupoint control group were treated by stimulating the fixed non-point under the bilateral rib, while mice in model control group and normal control group were raised without special treatment but administered the stimulation of catching with the same time and the same stimulus intensity. All treatrment lasted for 8 weeks. After the intervention, the learning and memory abilitities and brain hippocampus Brd U positive cells of all groups were detected. Results Compared with the nomal control group, mice in the model control group had longer escape latency and less time spent in former platform quadrant（P0.05）; the number of Brd U positive cells decreased significantly（P0.05）. Compared with the model control group and the non-acupoint control group, mice in the acupuncture group had shorter escape latency and more time spent in former platform quadrant（P0.05）; the number of Brd U positive cells increased significantly（P0.05）. Conclusion Bushen Huoxue acupuncture method can improve the learning and memory abilities of SAMP8 mice AD model by inducing the proliferation of hippocampal endogenous NSCs.

关 键 词：补肾活血 针刺 阿尔茨海默病 神经干细胞增殖 SAMP8小鼠 

分 类 号：R245[医药卫生—针灸推拿学]