多指标正交试验优化芪蛭益肺颗粒水提醇沉工艺  

Optimization of Water Extraction and Alcohol Precipitation Process of Qizhi Yifei Granules by Multi-Index Orthogonal Experimental

作　　者：王文平[1] 倪健[1] 冷新[1] 杨春静[1] 游龙泰 刘艺 塞那 梁潇 尹兴斌[1] WANG Wen-ping;NI Jian;LENG Xin;YANG Chun-jing;YOU Long-tai;LIU Yi;SAI Na;LIANG Xiao;YIN Xing-bin(College of Pharmacy,Beijing University of Chinese Medicine,Beijing 100102,China;Shanghai Binuo Medical Instrument Co.,Ltd.,Shanghai 201414,China)

机构地区：[1]北京中医药大学中药学院,北京100102 [2]上海弼诺医疗器械有限公司,上海201414

出　　处：《中国中医药信息杂志》2018年第9期71-75,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：北京市科技计划"十病十药"研发(Z020071620099)

摘　　要：目的采用多指标正交试验优选芪蛭益肺颗粒的最佳提取及醇沉工艺。方法以黄芪中黄芪甲苷、葶苈子中槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷的提取率及干膏得率为指标,优选芪蛭益肺颗粒的提取工艺;以黄芪甲苷、槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷的保留率为指标,优选最佳醇沉工艺。结果最佳水提工艺为:加10倍量水,提取1.5 h,共提取3次。最佳醇沉工艺为:浓缩至相对密度1.05~1.10(60℃),加乙醇至60%醇沉。结论本试验优选的水提醇沉工艺稳定可行,可为该制剂生产提供依据。Objective To optimize the extraction and alcohol precipitation process of Qizhi Yifei Granules by multi index orthogonal experiment. Methods With extraction rate of astragaloside in Astragali Radix, quercetin-3-O-β-D-glucose-7-O-β-D-gentian diglucoside in Descurainiae Semen Lepidii Semen and yield rate of dry extract as indexes, the extraction process of Qizhi Yifei Granules was optimized. Taking the retention rate of astragaloside and quercetin-3-O-β-D-glucose-7-O-β-D-gentian diglucoside as indexes, the alcohol precipitation process was optimized. Results The best water extraction process was as follows： adding 10 times amount of water, extracting for 1.5 h, 3 times. The optimum alcohol precipitation process was： concentrated to the relative density of 1.05–1.10（60 ℃）, adding ethanol to 60% and alcohol precipitation. Conclusion The optimized extraction and alcohol precipitation process is stable and feasible, which can provide the basis for the preparation.

关 键 词：芪蛭益肺颗粒 水提醇沉 黄芪甲苷 槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷 

分 类 号：R283.5[医药卫生—中药学]