不同产地半夏二氧化硫残留量、浸出物及主要化学成分含量测定  

Content Ditermination of Sulfer Dioxide Residues, Extracts and Main Chemical Compositions in Pinelliae Rhizoma from Different Producting Areas

作　　者：朱育凤[1] 邱明鸣 王迅[1] 朱健强 黄亚威 ZHU Yu-feng;QIU Ming-ming;WANG Xun;ZHU Jian-qiang;HUANG Ya-wei(Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China)

机构地区：[1]南京中医药大学附属医院,江苏南京210029

出　　处：《中国中医药信息杂志》2018年第9期79-82,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：江苏省中医药局科技项目(YB2017025)

摘　　要：目的测定半夏中二氧化硫残留量、浸出物及主要化学成分,探讨硫磺熏蒸对半夏性状、浸出物及指标性成分的影响。方法采用RP-HPLC测定不同产地22份半夏样品中琥珀酸、腺苷、鸟苷的含量,采用冷浸法测定半夏的浸出物,采用酸碱滴定法测定半夏二氧化硫残留量。结果 22份半夏样品中,硫磺熏蒸的样品占81.8%;琥珀酸、腺苷、鸟苷3个被测组分分离度良好,达到基线分离,平均加样回收率分别为99.1%、100.1%、99.3%。结论半夏产地加工硫磺熏蒸现象严重,硫磺熏蒸对半夏的性状、浸出物、琥珀酸含量均有一定程度的影响。本研究为2020年版《中华人民共和国药典》半夏质量标准修订提供了参考依据。Objective To determine the content of sulfur dioxide residues, extracts and main components; To study the effects of sulfur fumigation on the characters, extracts and index components of Pinelliae Rhizoma. Methods The contents of succinic acid, adenosine and guanosine in 22 samples of Pinelliae Rhizoma from different producing areas were detected by RP-HPLC method. The extracts of Pinelliae Rhizoma were measured by cold-maceration. Sulfur dioxide residues in Pinelliae Rhizoma were examined by acid-base titration. Results Among the 22 samples of Pinelliae Rhizoma, the ratio of sulphur smoked products of Pinelliae Rhizoma was 81.8%. Three kinds of components of succinic acid, adenosine and guanosine were well separated, baseline separation was achieved, and the average recoveries of samples were 99.1%, 100.1% and 99.3%, respectively. Conclusion The phenomenon of sulfur fumigation is very serious in feild processed products of Pinelliae Rhizoma. To some extent, sulfur fumigation has an influence on the characters, extracts and the content of succinic acid in Pinelliae Rhizoma. This study provides references for quality standard revision of Pinelliae Rhizoma in Chinese Pharmacopoeia 2020.

关 键 词：半夏 二氧化硫残留量 浸出物 含量测定 

分 类 号：R284.1[医药卫生—中药学]