肠安Ⅰ号方不同提取工艺对小鼠镇痛止泻作用影响的观察  

Effects of different extraction techniques of Chang'an Ⅰ decoction on the analgesic and antidiarrheal effects of mice

作　　者：郑蕊[1] 刘宇[1] 王凤云[2] 唐旭东[2] 马祥雪 石啸双 孙巧红[1] 金静 ZHENG Rui;LIU Yu;WANG Fengyun(Laboratory of Traditional Chinese Medicine Preparation,Xiyuan Hospital of Chinese Academy of Chinese Medical Sciences,Beijing 100091)

机构地区：[1]中国中医科学院西苑医院中药制剂研究室,北京100091 [2]中国中医科学院西苑医院脾胃病研究所,北京100091

出　　处：《河北中医》2018年第8期1217-1222,共6页Hebei Journal of Traditional Chinese Medicine

基　　金：北京市科学技术委员会G20工程支撑保障项目(编号:Z151100003815023)

摘　　要：目的观察比较肠安Ⅰ号方不同提取工艺对小鼠镇痛止泻作用的影响。方法分别采取6种不同提取工艺提取肠安Ⅰ号方药物成分,再分别通过镇痛实验(醋酸扭体反应)、止泻实验(蓖麻油致泻)观察其对小鼠镇痛、止泻的效果。镇痛实验:取小鼠80只,随机分为空白模型组、阿司匹林组、提取物Ⅰ组、提取物Ⅱ组、提取物Ⅲ组、提取物Ⅳ组、提取物Ⅴ组及提取物Ⅵ组,每组10只。空白模型组予纯化水灌胃(20 m L/kg),阿司匹林组予阿司匹林肠溶片药液灌胃(26 mg/kg),肠安Ⅰ号方提取物各组分别予对应提取工艺的药液灌胃(15.47 g/kg)。各组小鼠均第5 d末次给药20 min后,腹腔注射0.6%醋酸溶液,观察各组小鼠发生扭体反应的次数,记录发生初次扭体的时间,计算扭体发生率和镇痛率。止泻实验:取小鼠90只,随机分为空白对照组、空白模型组、马来酸曲美布汀组、提取物Ⅰ组、提取物Ⅱ组、提取物Ⅲ组、提取物Ⅳ组、提取物Ⅴ组及提取物Ⅵ组,每组10只。空白对照组和空白模型组予纯化水灌胃(20 m L/kg),马来酸曲美布汀组予马来酸曲美布汀胶囊药液灌胃(78 mg/kg),肠安Ⅰ号方提取物各组分别予对应提取工艺的药液灌胃(15.47 g/kg)。各组小鼠均连续灌胃7 d,除空白对照组外其余各组均于末次灌胃1 h后予蓖麻油灌胃,观察小鼠排便情况,分别计算蓖麻油作用0~1、1~2、2~3、3~4及0~4 h的腹泻指数。结果初次扭体时间比较,仅提取物Ⅰ组及阿司匹林组初次扭体时间均明显晚于空白模型组(P<0.05),其中提取物Ⅰ组小鼠初次扭体时间与阿司匹林组相当(P>0.05);扭体次数比较,仅提取物Ⅰ组、提取物Ⅲ组、取物Ⅴ组及阿司匹林组扭体次数均明显少于空白模型组(P<0.05),其中提取物Ⅰ组、提取物Ⅲ组及提取物Ⅴ组小鼠扭体次数与阿司匹林组相当(P>0.05);腹泻指数比较,空白模型组在各时间段腹泻指数均明显高于�Objective To observe the clinical effects of different extraction techniques of Chang＇anⅠdecoction on the analgesic and antidiarrheal effects of mice.MethodsSix different extraction techniques were used to extract the ingredients of Chang＇anⅠ,and the effects of analgesic and antidiarrheal on mice were observed by analgesic test（acetate writhing reaction）and antidiarrheal test（castor oil diarrhea）.Analgesic experiment：80 mice were randomly divided into blank model group,aspirin group,extract groupⅠ,extract groupⅡ,extract groupⅢ,extract groupⅣ,extract groupⅤand extract groupⅥ（n=10）.The blank model group was given purified water by gavage（20 m L/kg）,the aspirin group was given aspirin enteric-coated tablets by gavage（26 mg/kg）,and the extracts of the Chang＇an I decoction were respectively given to the corresponding extraction by gavage（15.47 g/kg）.The mice in each group were injected 0.6%acetic acid solution into abdominal cavity 20 minutes after the last administration on the5th day.The number of writhing reactions,the time of the first writhing,the incidence of writhing and the analgesic rate were observed.Antidiarrheal experiment：90 mice were randomly divided into blank control group,blank model group,trimebutine maleate group,extract groupⅠ,extract groupⅡ,extract groupⅢ,extract groupⅣ,extract groupⅤand extract groupⅥ（n=10）.The blank control group and the blank model group was given purified water by gavage（20 m L/kg）,the trimembutine maleate group was given trimembutine maleate capsule solution by gavage（78 mg/kg）,and the extracts of the intestine I extract were respectively administered to the corresponding extraction process,and the extracts of the Chang＇an I decoction were respectively given to the corresponding extraction by gavage（15.47 g/kg）.The mice in each group were fed with castor oil for 7 days.Except for the blank control group,the other groups were fed with castor oil for 1 hour after the last gastric administration.The

关 键 词：肠易激综合征 镇痛 止泻 动物 实验 小鼠 中药疗法 中药制药工艺 

分 类 号：R256.34[医药卫生—中医内科学] R284.2[医药卫生—中医学]