冀谷34中自噬相关基因SiATG5的克隆及响应干旱胁迫机理研究  

Cloning and Function Analysis of Autophagy Related Gene SiATG5 in Foxtail Millet(Setaria italic)Variety Jigu 34 under Drought Stress

作　　者：王根平[1] 刘敏轩 张婷[1] 师志刚[1] 张喜瑞[1] 杨伟红[1] 李琳[1] 高翔[1] 董立[1] 程汝宏[1] WANG Gen-ping;LIU Min-xuan;ZHANG Ting;SHI Zhi-gang;ZHANG Xi-rui;YANG Wei-hong;LI Lin;GAO Xiang;DONG Li;CHENG Ru-hong(Institute of Millet Crops of Hebei Academy of Agriculture and Forestry Sciences,National Foxtail Millet Improvement Center,Cereal Crops Laboratory of Hebei Province,Shijiazhuang 050035,China;Institute of Crop Sciences of Chinese Academy and Genetic Improvement,Beijing of Agricultural Sciences,The National Key Facility for Crop Gene Resources 100081,China)

机构地区：[1]河北省农林科学院谷子研究所国家谷子改良中心河北省杂粮研究实验室,河北石家庄050035 [2]中国农业科学院作物科学研究所国家农作物基因资源与基因改良重大科学工程,北京100081

出　　处：《河北农业科学》2018年第2期1-5,11,共6页Journal of Hebei Agricultural Sciences

基　　金：国家谷子糜子产业技术体系项目(CARS-06-13.5-A3);河北省农林科学院青年基金项目(A2015030102);河北省农林科学院博士基金项目(F17E02);河北省科技计划项目(16227508D-8);北京市科委课题(Z161100000916003);河北省财政专项(F17R05);河北省自然科学基金项目(C2017301085)

摘　　要：自噬相关基因ATG5是植物应答逆境胁迫的一个关键基因。谷子SiATG5响应干旱的作用模式尚不明确。研究干旱胁迫下谷子中ATG5的表达模式,可揭示SiATG5在响应谷子抗旱中的作用。以谷子抗旱品种冀谷34为试材,克隆SiATG5;研究轻度干旱(PEG-6000浓度6%)、中度干旱(PEG-6000浓度18%)和重度干旱(PEG-6000浓度30%)胁迫下,SiATG5在冀谷34中的表达模式。结果表明:SiATG5全长1 092 bp,无内含子,编码363个氨基酸。预测分子量为40.3 kD,理论等电点为5.03,三级结构分析表明含有ATG5特有结构域,YLoc软件预测位于细胞质内。其SiATG5与高粱和玉米中的ATG5同源性最高。SiATG5在冀谷34中的表达结果分析表明,在轻度和中度胁迫下24 h内表达量逐渐升高;重度胁迫下6 h表达量达到最高,随后逐渐降低。中、重度胁迫下的表达量显著高于轻度胁迫下的表达量(P<0.05)。SiATG5参与冀谷34的抗旱反应,为研究SiATG5在响应干旱胁迫中的作用及冀谷34抗旱机理奠定了基础。SiATG5 related to autophagy is a key gene in response to abiotic stress in plant. The functions of SiATG5 in foxtail millet in response to drought stress have little report. The study of SiATG5 expression patterns under different drought stress can reveal its function in response to drought in foxtail millet. SiATG5 gene was cloned from drought-resistant foxtail millet variety Jigu 34 and its expression patterns under different drought conditions with PEG-6000 of mild drought（6% PEG-6000）, moderate drought（18% PEG-6000） and severe drought（30% PEG-6000） were studied. The results showed that SiATG5 gene had 1 092 bp without intron coding 363 amino acids, the forecasted molecular weight of SiATG5 was 40.3 k D, with theory isoelectric point of 5.03. Tertiary structure analysis indicated that it contained specific domain of ATG5 protein, which located in the cytoplasm predicted by YLoc software. The homology of SiATG5 to sorghum and corn was the highest. Under mild and moderate drought stress,the expression level of SiATG5 gradually increased within 24 hours, while that was the highest after 6 hours under severe drought stress,then gradually reduced. The expression level of SiATG5 was significantly higher under moderate and severe drought stress than that under mild drought stress（P〈0.05）. The results indicated that SiATG5 gene involved in drought resistance reaction of Jigu 34 and played an important role in response to drought stress. The research laid the foundation for studying the function of SiATG5 under drought stress and drought resistance mechanism of Jigu 34.

关 键 词：自噬基因 抗旱 SiATG5 冀谷34 

分 类 号：S515[农业科学—作物学]