青藤碱对胰腺癌细胞系SW1990增殖及凋亡的影响  

Effect of Sinomenine on Pancreatic Cancer Cell SW1990 Proliferation and Apoptosis

作　　者：朱昌毫 孙诚谊[1] 喻超[1] 田舍[1] 陈世裕 张乙凡 潘耀振[1] ZHU Changhao;SUN Chengyi;YU Chao;TIAN She;CHEN Shiyu;ZHANG Yifan;PAN Yaozhen(Department of Hepatobiliary Surgery,the Ajfiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学附院肝胆外科,贵州贵阳550004

出　　处：《贵州医科大学学报》2018年第8期879-883,共5页Journal of Guizhou Medical University

基　　金：国家国际科技合作专项基金(2014DFA31420);国家自然科学基金资助项目(81660483);贵州省科学技术基金[黔科合J字(2015)2013号];贵州省第四批人才基地基金[黔省专合字(2012)94号];贵州省科学技术厅-贵阳医学院院士工作站肝胆外科分站[黔科合院士站(2015)4013];贵州医科大学肝胆胰脾重点实验室项目

摘　　要：目的:探讨青藤碱(SIN)对胰腺癌细胞增殖及凋亡的影响。方法:胰腺癌SW1900细胞分为实验组和对照组,实验组设置SIN浓度梯度0.125、0.25、0.5、1.0及2.0 mmol/L组,对照组不作处理;CCK-8方法检测SIN对胰腺癌细胞SW1990增殖的影响,平板克隆实验检测SIN对胰腺癌SW1990细胞集落形成的影响,流式细胞术检测SIN对胰腺癌SW1990细胞凋亡的影响,Western blot检测SIN对胰腺癌SW1990细胞中PARP蛋白表达水平的影响。结果:SIN作用于SW1990细胞后,细胞增殖率随着SIN剂量增加而降低(P<0.05),细胞集落形成数量逐渐减少(P<0.05),细胞凋亡率逐渐增加(P<0.05);相同浓度SIN时,随着作用时间延长,其对SW1990细胞增殖的抑制作用逐渐增强(P<0.05);Western blot结果显示胰腺癌SW1990细胞PARP蛋白表达水平逐渐随着SIN剂量增加而减少(P<0.05)。结论:青藤碱能有效抑制胰腺癌SW1990细胞的增殖,其机制可能与诱导SW1990细胞凋亡有关。Objective： To investigate the effect of Sinomenine on proliferation and apoptosis of pancreatic cancer cell SW1990. Methods： Human pancreatic cancer cell line SW1990 cells were divided into experiment group and control group; experiment group was further divided into 0. 125,0. 25,0. 5,1. 0 and 2. 0 mmol/L group by concentrations of SIN,while control group receive none treatment.CCK-8,plate cloning experiment,flow cytometry and Western blot were adopted to test the effect of SIN on pancreatic cancer cell SW1990 proliferation activity,colony formation,apoptosis,and protein expression level of PARP respectively. Results： Experiment showed that cell proliferation rate decreased as the SIN dosage increased（ P〈0. 05）,colony formation decreased gradually（ P〈0. 05）,apoptosis rate increased gradually（ P〈0. 05）; with the same concentration level,as the time prolonged,the inhibition effect on SW1990 cell proliferation enhanced（ P〈0. 05）; Western blot showed that protein expression level of PARP gradually increased as the SIN dosage added（ P〈0. 05）. Conclusion： These results suggest that Sinomenine could inhibit proliferation and induce apoptosis in human pancreatic cancer cell SW1990.

关 键 词：胰腺肿瘤 癌 青藤碱 增殖 凋亡 SW1990细胞 

分 类 号：R285.5[医药卫生—中药学] R735.9[医药卫生—中医学]