海藻酸钠固定化菌株JY03及其降酚特性分析  

Study on Immobilization of Phenol-degrading Strain JY03 and Its Degradation Performance

作　　者：姜立春[1] 赵莉[1] 杨苹 孙翘 阮期平[1] JIANG Lichun;ZHAO Li;YANG Ping;SUN Qiao;RUAN Qiping(Key Laboratory for Molecular Biology and Biopharmaceutics,Mianyang Teachers College,Mianyang,Sichuan 621006)

机构地区：[1]绵阳师范学院分子生物学与生物制药重点实验室,四川绵阳621006

出　　处：《绵阳师范学院学报》2018年第8期5-11,共7页Journal of Mianyang Teachers＇ College

基　　金：四川省科技厅项目(2016JY0038);绵阳市应用基础研发项目(17YFNY007);绵阳师范学院科研项目(PY-2016-A03;MNU-JY16162)

摘　　要：以海藻酸钠作为包埋载体,对苯酚降解菌株JY03进行细胞固定化,并对降解条件进行了优化分析.通过单因素试验确定了合适的固定化条件为:海藻酸钠浓度2.5%,CaCl_2浓度3.0%,包埋菌体量0.3 g,钙化时间8 h.固定化细胞降解苯酚的最适条件为:温度32℃,pH值6.5,接种量30 g,对苯酚浓度800 mg/L降解36 h,其降解率达到88%以上.菌株JY03通过海藻酸钠固定化对苯酚的降解能力与耐受能力均大于游离细胞,这将为进一步采用固定化微生物方法处理含酚废水提供了试验依据.In this paper,the phenol-degrading strain JY03 was immobilized on sodium alginate. The optimal immobilization conditions were determined by single factor experiment as fllow： sodium alginate of 2. 5 %,CaCl_2 of 3. 0 %,amount of wet strain of 0. 3 g,calcification time of 8 h. The optimum conditions for immobilized cell degrading phenol were as follows： temperature of 32 ℃,pH value of 6. 5,inoculation amount of 30 g. The results showed that the degradation performance and tolerance of immobilized cells for phenol were obviously better than those of free cells. When 800 mg·L^（-1） of phenol was degraded by immobilized cell for 36 h,its degradation rate reached over 88 %. This will provide an experimental basis for the further use of immobilized microorganism to treat phenol containing waste-water.

关 键 词：苯酚 海藻酸钠 Corynebcterium sp. JY03 固定化 生物降解 

分 类 号：Q935[生物学—微生物学] X703.1[环境科学与工程—环境工程]