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作 者:郭晓慧 郭雅琼[1] 李扬[1] Guo Xiao-Hui;Guo Ya-Qiong;Li Yang(Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring,Guangdong Provincial Key Laboratory of Healthy and Safe Aquaculture,College of Life Science,South China Normal University,Guangzhou 510631,China)
机构地区:[1]华南师范大学生命科学学院广州市亚热带生物多样性与环境生物监测重点实验室广东省水产健康安全养殖重点实验室,广州510631
出 处:《植物科学学报》2018年第4期508-517,共10页Plant Science Journal
基 金:国家自然科学基金(31570205;31370235);广州市科技计划项目(201607010370)资助~~
摘 要:采用毛细管复洗技术建立海链藻属(Thalassiosira)物种的单克隆培养株系,结合形态学和分子系统学研究,对小字海链藻原变种(Thalassiosira minuscula var.minuscula Krasske)的形态特征进行观察,并报道了1个新变种:小字海链藻双卫变种(T.minuscula var.bicustodis X.H.Guo,Y.Q.Guo&Y.Li)。双卫变种与原变种的形态特征基本一致,区别仅在于唇形突的伴生支持突数目不同,双卫变种具有2个,原变种只有1个。基于对多个株系生活史的连续观察,确认其伴生支持突的数目稳定,具有分类学意义。To clarify the species diversity of the genus Thalassiosira,monoclonal strains were isolated and established from the Chinese coast. Morphology was observed using light and electron microscopy and sequences of small and large subunit nuclear ribosomal encoding genes were obtained to build phylogenetic relationships. Among 10 established T. minuscula Takano strains, morphological variations and corresponding molecular divergences were found. After comparison with the original description of T. minuscula, specimens with a rimoportula neighboring one fultoportula were assigned as T. minuscula var. minuscula,and those with a rimoportula neighboring two fultoportulae were proposed as a new variety,T.minuscula var. bicustodis X. H. Guo,Y. Q. Guo Y. Li. On the phylogenetic trees inferred from small and large subunit nuclear ribosomal encoding genes,T. minuscula var. minuscula was clustered with T. minuscula var. bicustodis as a sister group(BPP 〉0.90),thus showing their close relationship. These results,combined with the morphological and molecular data,support the erection of T. minuscula var. bicustodis.
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