基于生物信息学分析肺炎链球菌LytR蛋白特征  

Study on characteristics of Streptococcus pneumoniae LytR protein based on bioinformatics

作　　者：吴凯峰[1] 张競之 查何 刘雁[1] 童华波[1] 潘卫[2] 王正蓉 WU Kaifeng1, ZHANG Jingzhi1, ZHA He1, LIU Yan1, TONG Huabo1, PAN Wei2, WANG Zhengrong2(1. Department of Clinical Laboratory, the First People＇ s Hospital of Zunyi/Third Affiliated Hospital of Zunyi Medical University, Zunyi , Guizhou 563000, China ; 2. College of Laboratory Medicine, Guizhou Medical University, Guiyang , Guizhou 550004, Chin)

机构地区：[1]贵州省遵义市第一人民医院/遵义医学院第三附属医院检验科,563000 [2]贵州医科大学检验医学院,贵阳550004

出　　处：《重庆医学》2018年第24期3125-3128,3133,共5页Chongqing medicine

基　　金：国家自然科学基金资助项目(31600115;81560324)

摘　　要：目的研究肺炎链球菌spr1759(LytR)蛋白的基本理化性质,初步分析预测该蛋白的结构与功能。方法利用Bioedit、TMHMM、TargetP和ExPASy等多种生物信息学软件对肺炎链球菌spr1759蛋白进行分析,并对其功能预测。利用TMHMM软件对比肺炎链球菌spr1759蛋白与炭疽芽孢杆菌BAS5115蛋白和枯草芽孢杆菌DJ97_1211二级结构差异;利用Megalign软件分析它们之间的氨基酸序列相似性。结果肺炎链球菌spr1759蛋白为一亲水性蛋白,其相对分子质量为37.56×10~3,等电点为5.11,存在1个跨膜区。肺炎链球菌spr1759蛋白属于LytR_cpsA_psr超家族,可能具有阴离子细胞壁多聚物合成相关酶的功能。与其他细菌蛋白比对发现,该蛋白与炭疽芽孢杆菌BAS5115蛋白和枯草芽孢杆菌DJ97_1211具有相似的跨膜结构域,均含有1跨膜结构;肺炎链球菌spr1759蛋白与炭疽芽孢杆菌BAS5115蛋白和枯草芽孢杆菌DJ97_1211蛋白氨基酸序列具有较高同源性,全长序列相似性分别为35.7%和32.5%。结论肺炎链球菌LytR蛋白是具有跨膜结构的亲水性蛋白,可能具有阴离子细胞壁多聚物合成相关酶学功能。Objective To study the basic physicochemical properties of Streptococcus pneumoniae spr1759（LytR）protein and to preliminarily analyze its structure and function.Methods Streptococcus pneumoniae spr1759 protein was analyzed by using multiple bioinformatic softwares including Bioedit,TMHMM,TargetP and ExPASy,and its functions were predicted.The secondary structure differences were compared between Streptococcus pneumoniae spr1759 protein and Bacillus anthracis BAS5115 protein or Bacillus subtilis DJ97_1211 protein by using the TMHMM software.The amino acid sequence similarity between them was analyzed by using the Megalign software.Results Streptococcus pneumoniae spr1759 protein was a hydrophilic protein with a relative molecular weight of 37.56×103,an isoelectric point of 5.11,and one transmembrane region.Streptococcus pneumoniae Spr1759 belonged to the LytR_cpsA_psr superfamily and might have the function of anionic cell wall polymer synthesis related enzyme.Protein structure comparison revealed that this protein had a transmembrane structure domain similar to that of Bacillus anthracis BAS5115 protein and Bacillus subtilis DJ97_1211,and all contained one transmembrane structure.Streptococcus pneumoniae spr1759 protein had higher homology with the amino sequence of Bacillus anthracis BAS5115 protein and Bacillus subtilis DJ97_1211,and the full length sequence similarities were 35.7% and 32.5%,respectively.Conclusion Streptococcus pneumoniae LytR protein is a hydrophilic protein with one transmembrane structure,which may have the function of anionic cell wall polymer synthesis related enzyme.

关 键 词：肺炎 链球菌属 肺炎链球菌 LytR蛋白 生物学信息学分析 

分 类 号：R378.14[医药卫生—病原生物学]