重组hIL-10联合威灵仙总皂苷对AA大鼠关节影像的影响及机制  

Effect and mechanism of recombinant hIL-10 combined with bauhinia championii benth flavones on the imaging findings of AA rats

作　　者：王斌 张燕 金苏华 珍妮 WANG Bin;ZHANG Yan;JIN Suhua;ZHEN Ni(Department of Medical Imaging;Department of Ophthalmology;Department of Medical ImagingMRI Room,Inner Mongolia Forestry General Hospital,Yakeshi,Inner Mongolia 022150,China)

机构地区：[1]内蒙古林业总医院医学影像科,内蒙古牙克石022150 [2]内蒙古林业总医院眼科,内蒙古牙克石022150 [3]内蒙古林业总医院医学影像科核磁共振室,内蒙古牙克石022150

出　　处：《重庆医学》2018年第23期3035-3038,共4页Chongqing medicine

基　　金：2017年内蒙古自然科学基金资助面上项目(2017MS0881)

摘　　要：目的分析重组hIL-10联合威灵仙总皂苷对佐剂性关节炎大鼠(AA大鼠)关节影像学表现的影像及其影响的机制。方法将150只大鼠分为空白组、对照组、观察组1、观察组2、观察组3。空白组不接受任何干预。对照组仅接受关节炎模型建立干预。3个观察组大鼠除接受佐剂型关节炎模型建立干预外,分别接受威灵仙总皂苷治疗,重组hIL-10治疗和重组hIL-10联合威灵仙总皂苷治疗。于实验第0、15、35天分别行X线检查关节间隙和关节评分;35d后处死大鼠,取动脉血检测其淋巴细胞和血清炎症因子。结果实验15d后对照组和3个观察组骨侵蚀关节侵蚀评分显著高于空白组,关节间隙显著减小(P<0.05);实验35d后观察组小鼠骨侵蚀关节侵蚀评分显著低于对照组,关节间隙显著大于对照组(P<0.05)。观察组3CD4+T细胞、CD4+/CD8+、IL-17A、IL-6和TNF-α显著低于对照组和观察组1、2(P<0.05)。结论重组hIL-10联合威灵仙总皂苷可以通过调节免疫有效保护AA大鼠关节。Objective To analyze the effect and mechanism of recombinant hIL-10 combined with bauhinia championii benth flavones on the imaging findings of adjuvant arthritis （AA） rats. Methods A total of 150 rats were divided into the blank group,the control group,the first observation group,the second observation group,the third observation group.The blank group did not receive any intervention.The control group only received AA model establishment intervention.In addition to the intervention of the AA model,the three observation groups respectively accepted the treatment of bauhinia championii benth flavones,recombinant hIL-10 and recombinant hIL-10 combined with bauhinia championii benth flavones.In the zeroth,15th,35th days of the experiment,the rats were underwent X-ray examination of joint space and joint score,and they were executed in the 35th day,the lymphocyte and serum inflammatory factors were detected from the arterial blood. Results After 15 d,the bone erosion scores of the control group and the three observation groups were significantly higher than that of the blank group,and the joint space decreased significantly （ P 〈0.05）.After 35 d,the bone erosion scores in the observation groups were significantly lower than that of the control group,and the joint space was significantly greater than that of the control group （ P 〈0.05）.The levels of CD4 ＋T cells,CD4 ＋/CD8 ＋,IL-17A,IL-6 and TNF-α in the third observation group were significantly lower than those in the control group and the first,second observation group （ P 〈0.05）. Conclusion Recombinant hIL-10 combined with bauhinia championii benth flavones can effectively protect the joints of AA rats by regulating the immune system.

关 键 词：重组hIL-10 威灵仙总皂苷 AA大鼠 影像学变化 

分 类 号：R593.22[医药卫生—内科学]