SIRT1对牛卵泡颗粒细胞凋亡及雌激素分泌的影响  

Effects of SIRT1 on Apoptosis and Estrogen Secretion of Bovine Granulosa Cells

作　　者：陈洋[1] 单雪松[1] 吕文发[1] 赵志辉[2] CHEN Yang;SHAN Xue-song;LV Wen-fa;ZHAO Zhi-hui(College of Animal Science and Technology,Jilin Agricultural University,Jilin Changchun 130118,China;College of Animal Science and Veterinary Medicine,Jilin University,Jilin Changchun 130062,China)

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国畜牧杂志》2018年第8期56-60,共5页Chinese Journal of Animal Science

基　　金："863"计划资助项目(2013AA102505)

摘　　要：本实验旨在研究沉默信息调节因子1(SIRT1)对牛卵泡颗粒细胞凋亡及雌激素分泌的影响。构建SIRT1基因干扰载体,并转染体外培养的颗粒细胞。实验分为转染SIRT1-sh RNA的干扰组、转染NC-sh RNA的对照组和未转染的空白组,每组设3个重复。流式细胞术检测细胞凋亡;实时荧光定量PCR检测细胞内Bax、Bcl-2 m RNA表达;Western Blot检测细胞内Bax、Bcl-2蛋白表达;ELISA试剂盒检测雌激素水平。结果表明:干扰组颗粒细胞SIRT1 m RNA表达量显著低于对照组与空白组(P<0.05),而对照组与空白组之间差异不显著(P>0.05);干扰组颗粒细胞凋亡率显著低于对照组与空白组(P<0.05);干扰组颗粒细胞Bax m RNA和蛋白质表达显著低于对照组与空白组(P<0.05),而Bcl-2 m RNA和蛋白质表达均显著高于对照组与空白组(P<0.05);干扰组颗粒细胞培养液中雌激素含量显著低于对照组与空白组(P<0.05)。SIRT1基因可以促进牛卵泡颗粒细胞的凋亡,并促进雌激素分泌。The purpose of this study was to investigate the effect of SIRT1 on apoptosis and estrogen secretion of bovine follicular granulosa cells. SIRT1 gene interference vectors were constructed and transfected into cultured granulosa cells. Experiments were divided into interference group transfected with SIRT1-sh RNA, control group transfected with NC-sh RNA, and untransfected blank group, with 3 replicates in each group. Flow cytometry was used to detect apoptosis. Real-time fl uorescence quantitative PCR was used to detect the expression of Bax and Bcl-2 m RNA. Western blot was used to detect the protein expression of Bax and Bcl-2. ELISA kit was used to detect estrogen levels. The results showed that the expression of SIRT1 m RNA in the granulosa cells in the interference group was signifi cantly lower than that in the control group and the blank group（P0.05）, while there was no signifi cant difference between the control group and the blank group（P0.05）. The apoptosis rate of granulosa cells in the interference group was signifi cantly lower（P0.05）. The expression of Bax m RNA and protein in the granulosa cells of the control group was signifi cantly lower than that of the control group and the blank group, while the expression of Bcl-2 m RNA and protein was signifi cantly higher than that of the control group and the blank group（P0.05）. The content of estrogen in the granulosa cell culture medium of the interference group was signifi cantly lower than that of the control group and the blank group. SIRT1 gene promotes apoptosis in bovine granulosa cells and promotes estrogen secretion.

关 键 词：SIRT1基因 颗粒细胞 凋亡 雌激素 

分 类 号：S823.3[农业科学—畜牧学]