Necrostatin-1对脂多糖刺激的断奶仔猪血细胞分类计数和血液生化指标的影响  

Effect of Necrostatin-1 on Blood Differential Count and Blood Biochemical Measurements of Piglets Challenged with Lipopolysaccharide

作　　者：汪洋[1] 王秀英[1] 汪龙梅[1] 代兵 刘秀婷 刘玉兰[1] WANG Yang;WANG Xiu-ying;WANG Long-mei;DAI Bing;LIU Xiu-ting;LIU Yu-lan(Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Hubei Wuhan 430023,China;Zhejiang Goshine Test Technologies Co.,Ltd.,Zhejiang Hangzhou 310030,China)

机构地区：[1]武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023 [2]浙江国正检测技术有限公司,浙江杭州310030

出　　处：《中国畜牧杂志》2018年第8期80-86,共7页Chinese Journal of Animal Science

基　　金：国家自然科学基金面上项目(31772615);湖北省教育厅优秀中青年科技创新团队项目(T201508);杭州市钱江特聘专家(3122)

摘　　要：本试验旨在研究程序性坏死阻断剂Necrostatin-1(Nec-1)对脂多糖(LPS)刺激断奶仔猪血细胞分类计数和血液生化指标的影响。选用25头断奶仔猪,分为对照组[10%二甲基亚砜(DMSO)+生理盐水]、LPS组(10%DMSO+LPS)、0.33Nec-1组[0.33 mg/kg体重(BW)Nec-1+LPS]、1.0Nec-1组(1.0 mg/kg BW Nec-1+LPS)和3.3Nec-1组(3.3 mg/kg BW Nec-1+LPS)。注射10%DMSO或Nec-1 30 min后,对照组注射生理盐水,其他组注射100μg/kg BW LPS,分别在注射LPS或生理盐水4 h和24 h后采血液样品待测。结果表明:注射LPS 4 h后,0.33 mg/kg BW Nec-1、1.0 mg/kg BW Nec-1和3.3 mg/kg BW Nec-1显著缓解LPS刺激导致的血小板分布宽度升高(P<0.05);0.33 mg/kg BW Nec-1能显著缓解LPS刺激导致的血浆谷草转氨酶(AST)活性和AST/谷丙转氨酶(ALT)比例的升高(P<0.05),1.0 mg/kg BW Nec-1显著缓解血浆葡萄糖含量和肌酸激酶活性的降低(P<0.05);注射LPS 24 h后,0.33 mg/kg BW Nec-1、1.0 mg/kg BW Nec-1和3.3 mg/kg BW Nec-1可显著缓解LPS刺激导致的嗜酸性粒细胞比例的升高(P<0.05),并有缓解嗜酸性粒细胞绝对值升高的趋势(P<0.10);0.33 mg/kg BW Nec-1缓解了肌酸激酶活性的降低并提高了血浆总胆固醇和甘油三酯含量(P<0.05),1.0 mg/kg BW Nec-1有缓解LPS刺激导致的血浆AST/ALT升高的趋势(P<0.10),3.3 mg/kg BW Nec-1能显著缓解AST/ALT比例的升高(P<0.05)和葡萄糖的降低(P<0.05),并有缓解AST升高和总胆固醇降低的趋势(P<0.10)。以上结果表明,Nec-1对LPS刺激导致的免疫应激反应具有一定的缓解作用。The study was conducted to investigate the effects of Necrostatin-1（Nec-1） on blood cell differential count and blood biochemical measurements of piglets challenged with lipopolysaccharide（LPS）. Twenty-five piglets were randomly assigned into 5 groups： control; LPS; LPS＋0.33 Nec-1（0.33 mg/kg BW）; LPS＋1.0 Nec-1（1.0 mg/kg BW）; LPS＋3.3 Nec-1（3.3 mg/kg BW）. After injection of 10%DMSO or Nec-1 for 30 mins, piglets were injected with either LPS or saline. At 4 h or 24 h after LPS or saline injection, blood samples were taken. The results showed that at 4 h, 0.33 mg/kg BW Nec-1, 1.0 mg/kg BW Nec-1 and 3.3 mg/kg BW Nec-1 alleviated the decrease of platelet distribution width induced by LPS challenge（P0.05）. 0.33 Nec-1 alleviated the increases of aspartate transaminase（AST） and AST/alanine aminotransferase（ALT）（P0.05）, and 1.0 Nec-1 alleviated the decrease of blood glucose and creatine kinase（P0.05） induced by LPS challenge. At 24 h, Nec-1 alleviated the increase of eosinophil proportion（ mg/kg BW P0.05）, and had a tendency to alleviate the increase of eosinophil count（P0.10） induced by LPS challenge. 0.33 mg/kg BW Nec-1 alleviated the decrease of creatine kinase and increased total cholesterol and triglyceride levels（P0.05）. 1.0 Nec-1 had a tendency to alleviate the increase of AST/ALT induced by LPS challenge（P0.10）. 3.3 mg/kg BW Nec-1 alleviated the decrease of blood glucose and the increase of AST/ALT（P0.05）, and had a tendency to alleviate the decrease of AST and total cholesterol（P0.10） induced by LPS challenge. These results indicate that Nec-1 alleviated the stress response induced by LPS challenge.

关 键 词：Nec-1 LPS 血细胞分类计数 血液生化指标 仔猪 

分 类 号：S828.5[农业科学—畜牧学]