土人参Actin基因片段的克隆及序列分析  

Cloning and sequence analysis of Actin gene fragment from Talinum paniculatum Gaertn

作　　者：叶玉妍 陈晓[1] 杨礼香[1] YE Yu-yan;CHEN Xiao;YANG Li-xiang(College of Life Sciences,Guangzhou University,Guangzhou 510006,China)

机构地区：[1]广州大学生命科学学院,广东广州510006

出　　处：《广东农业科学》2018年第6期19-24,共6页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31570257)

摘　　要：为土人参功能基因的表达与调控提供内参基因,根据已知植物Actin基因的保守区设计简并性引物,采用RT-PCR方法扩增得到土人参的Actin基因片段,将该片段连接于p GEM-T载体,测序获得一段大小为598 bp的基因片段,该片段编码198个氨基酸。通过生物信息学软件分析,该序列与其他植物Actin基因的cDNA序列同源性均在85%以上,氨基酸序列的同源性达到86%以上,表明试验克隆所得基因片段为土人参Actin基因片段。将该Actin基因片段命名为TpActin1,并登陆在Gena Bank,登录号为MH333039。In order to provide reference gene for studying the expression pattern of functional genes in Talinum paniculatum,the cDNA fragment of Actin from Talinum paniculatum;was amplified by RT-PCR,by adopting degenerated primers designed according to the conserved region of the Actin genes which were submitted to NCBI database. The cDNA fragment was inserted into the p GEM-T vector and sequenced. The cDNA sequence was analyzed by online bioinformatics software. Our result showed that this actin cDNA includes 598 base pairs,encoding a partial actin with 198 amino acids. Sequence alignments showed that it shares over 85% nucleotide sequence identity and 86% amino acid sequence identity with other plant actins. This study indicated that a new actin cDNA fragment was isolated from Talinum paniculatum. This cDNA was named TpActin1 and submitted to GenBank （accession number： MH33309）.

关 键 词：土人参 肌动蛋白 ACTIN基因 克隆 序列分析 

分 类 号：S567.23[农业科学—中草药栽培]