益生菌干预对运动大鼠胃肠激素与AQP4表达的影响  

Effect of probiotics on gastrointestinal hormones and expression of AQP4 in rats during exercise

作　　者：莫伟彬[1,2] 杨衍滔 郭艳菊[1] 周彩华[1] 邵晓云 MO Weibin;YANG Yantao;GUO Yanju;ZHOU Caihua;SHAO Xiaoyun(Sport School of Guangxi Normal University,Guilin 541004,China;State Key Laboratory of Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources,Ministry of Science and Technology of China,Guilin 541004;Department of Human Anatomy,Guilin Medical University,Guilin 541004)

机构地区：[1]广西师范大学体育学院,广西桂林541004 [2]药用资源化学与药物分子工程国家重点实验室,广西桂林541004 [3]桂林医学院人体解剖学教研室,广西桂林541004

出　　处：《中国实验动物学报》2018年第4期411-417,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金资助项目(No.21562006;No.31060121);教育发展基金会"教师成长基金"项目(No.EDF2016005);广西教育厅科研项目(No.2016JGY73);国家重点实验室开放项目(No.CMEMR2012-B04)~~

摘　　要：目的研究益生菌干预下力竭性运动大鼠胃肠道功能的影响。方法 40只SD雄性大鼠随机分为安静对照组(NC),运动对照组(ME),安静给药组(NCY),运动给药组(MEY)。ME与MEY组进行6周力竭训练,速度为19.3 m/min,坡度为5o,每天训练1次,每周6 d。NCY与MEY组每日灌胃浓度为10~7CFU/mL益生菌溶液10.0 mL/(kg·d)。6周力竭训练结束后,检测大鼠血清中胃肠激素ghrelin、PYY、CCK、GLP-1、MTL和GAS指标含量,并采用RT-PCR和Western blot法检测大鼠胃和结肠组织中AQP4的表达。结果 ME组大鼠血清ghrelin和PYY含量均高于NC组(P<0.01),而ME组CCK、GLP-1、MTL和GAS含量均低于NC组(P<0.01);NCY组大鼠血清ghrelin、PYY均低于ME组(P<0.01),而CCK、GLP-1、MTL和GAS含量均高于ME组(P<0.01);MEY组大鼠血清ghrelin高于NC组(P<0.05)而低于ME组(P<0.01);MEY组大鼠血清PYY的含量低于ME组(P<0.01);MEY组大鼠血清CCK含量低于NC组(P<0.05)而高于ME组(P<0.05);MEY组大鼠血清GLP-1、MTL和GAS含量均高于ME组(P<0.01)。ME组大鼠胃肠组织AQP4 mRNA和蛋白表达均低于NC组(P<0.01或P<0.05);NCY和MEY组大鼠胃肠组织AQP4 mRNA和蛋白表达均高于ME组(P<0.01或P<0.05)。结论力竭性运动引起大鼠胃肠激素含量发生变化以及AQP4表达的上升与补充益生菌溶液密切相关,提示益生菌可能参与了胃肠激素的分泌和水代谢的过程。Objective To study the effect of intervention of probiotics on gastrointestinal function and expression of AQP4 in rats during exhaustive exercise. Methods Forty male SD rats were randomly divided into quiet control group（ NC）,exercise control group（ ME）,quiet medication group（ NCY） and exercise medication group（ MEY）. The exercise control group and the exercise medication group were subjected to exhaustive training for 6 weeks： the running speed was 19. 3 m/min,the slope was 5°,the training was once a day,6 d/week for 6 weeks. Probiotics in concentration of 107 CFU/mL was gavaged 10. 0 mL/（ kg/d） in the quiet medication group and exercise medication group. After 6 weeks of exhaustive training,the activities of serum gastrointestinal hormones ghrelin,PYY,CCK,GLP-1,MTL and GAS were measured.. The expression of AQP4 was detected by RT-PCR and western blot. Results The contents of serum ghrelin and PYY were significantly higher in the ME group than those in the NC group（ P〈 0. 01）. The contents of intestinal CCK,GLP-1,MTL and GAS in the ME group were significantly lower than those in the NC group（ P〈 0. 01）. The serum ghrelin and PYY were lower in the NCY group than in the ME group（ P〈 0. 01）,while the contents of intestinal CCK,GLP-1,MTL and GAS in the NCY group were higher than those in the ME group（ P〈 0. 01）. The serum ghrelin in the MEY group was higher than that in the NC group（ P〈 0. 05） and lower than that in the ME group（ P〈 0. 01）.The PYY in the MEY group was lower than that in the ME group（ P〈 0. 05）. The serum CCK was lower in the MEY group than that in the NC group（ P〈 0. 05） and higher than that in the ME group（ P〈 0. 05）. The serum GLP-1,MTL and GAS in the MEY group were higher than those in the ME group（ P〈 0. 01）. The expression of AQP4 mRNA and protein in the gastrointestinal tissue of the ME group were lower than those in the NC group（ P〈 0. 01 or P〈 0. 05）. The expression of AQP4 mRNA and protein in the NCY

关 键 词：益生菌 力竭运动 胃肠激素 水通道蛋白-4 大鼠 

分 类 号：Q95-33[生物学—动物学]