阿尔茨海默病转基因小鼠极早期嗅觉工作记忆的损害特征  

Olfactory working memory impairment in the transgenic mice at very early stage of Alzheimer's disease

作　　者：张记春 黄庚娣 孟雪[1] 田永路 于欣[1] 张晨[3] 王华丽[1] ZHANG Jichun;HUANG Gengdi;MENG Xue;TIAN Yonglu;YU Xin;ZHANG Chen;WANG Huali(Peking University Sixth Hospital,Peking University Institute of Mental Health,National Clinical Research Center for Mental Disorders,Beijing Dementia Key Lab,Beijing 100191,China;Center for Life Sciences,Academy for Advanced Interdisciplinary Studies,Peking University,Beijing 100871;School of Life Sciences,Peking University,Beijing 100871)

机构地区：[1]北京大学第六医院北京大学精神卫生研究所国家精神心理疾病临床医学研究中心痴呆诊治转化医学研究北京市重点实验室,北京100191 [2]北京大学前沿交叉学科研究院生命科学联合中心,北京100871 [3]北京大学生命科学学院,北京100871

出　　处：《中国实验动物学报》2018年第4期437-443,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：北京市科学技术委员会脑医学研究重点专项(No.Z161100002616021);国家重点研发计划项目(No.2017YFC1311100)~~

摘　　要：目的探讨阿尔茨海默病转基因小鼠极早期嗅觉工作记忆损害特征。方法选用5周龄雄性5XFAD转基因及野生型小鼠各9只,9周龄雄性5XFAD转基因与野生型小鼠各8只,采用嗅觉广度测验进行测试,以嗅觉广度、气味辨别错误数和准确率评估嗅觉工作记忆能力。结果与同龄野生型小鼠相比,9周龄转基因小鼠嗅觉广度明显降低(P<0.05),错误次数明显增高(P<0.05),气味辨别准确率显著降低(P<0.05)。此外,与5周龄转基因小鼠相比,9周龄转基因小鼠嗅觉广度明显降低(P<0.05)。而5周龄5XFAD小鼠的嗅觉广度、错误次数以及气味辨别准确率与野生型之间差异无显著性(P>0.05)。结论 9周龄5XFAD转基因小鼠出现显著嗅觉工作记忆损害,提示嗅觉工作记忆损害可能是AD小鼠模型极早期认知损害的敏感指标。Objective To explore the characteristic impairments of olfactory working memory in the transgenic mice at very early stage of Alzheimer＇s disease（ AD）. Methods Nine pairs of 5-week-old male 5 XFAD mice and their wild-type littermates,and 8 pairs of 9-week-old male 5 XFAD mice and their wild-type littermates were used. The study animals underwent the odor span test after extensive training. The odor span length,the number of errors and the accuracy rate of odor discrimination were used to evaluate the olfactory working memory. Results Compared with the wild-type group,the 9-week-old transgenic mice presented significantly shorter odor span length（ P〈 0. 05）,more errors（ P〈 0. 05） and lower accuracy（ P〈 0. 05） of odor discrimination. Besides,the 9-week-old transgenic mice exhibited significantly shorter odor span length than the 5-week-old transgenic mice（ P〈 0. 05）. However,there were no significant differences in the odor span length,the number of errors and the accuracy between the 5-week-old transgenic and wild-type mice（ P〉 0. 05）. Conclusions Olfactory working memory is impaired in the 9-week-old transgenic 5 XFAD mice. It suggests that the olfactory working memory impairment might be considered as a sensitive indicator of very early cognitive impairment in the 5 XFAD mice.

关 键 词：阿尔茨海默病 嗅觉广度 转基因小鼠 工作记忆 

分 类 号：Q95-33[生物学—动物学]