果蝇研究技术与资源的开发  

Research techniques and resource development of Drosophila melanogaster

作　　者：徐荣刚[1] 王霞[1] 王芳 孙锦[1] 毛德才 乔欢欢[1] 贾豫 朱芮葆 彭娉 刘鲁萍[1] 倪建泉 XU Ronggang;WANG Xia;WANG Fang;SUN Jin;MAO Decai;QIAO Huanhuan;JIA Yu;ZHU Ruibao;PENG Ping;LIU Luping;NI Jianquan(School of Medicine,Tsinghua University,Beijing 100084,China)

机构地区：[1]清华大学医学院,北京100084

出　　处：《中国实验动物学报》2018年第4期489-492,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家科技支撑计划(No.2015BAI09B03)~~

摘　　要：黑腹果蝇(Drosophila melanogaster)是进行生物医学研究的理想模型,但研究过程往往受到遗传工具和品系资源的限制,无法有效地调控目的基因表达,阻碍实验的深入开展。为了方便果蝇领域的实验室开展研究,我们首先开发并优化了基于CRISPR/Cas9的基因组编辑技术,随后研发了转录激活系统和新一代转基因干扰技术,最终可以简单高效、准确特异地调控目的基因表达。同时,利用这些遗传学新技术,我们构建了相应的基因敲除、转基因转录激活、转基因干扰的果蝇品系资源库,使清华大学果蝇中心成为世界上最重要的果蝇技术和资源中心之一。这些新技术以及相应的果蝇资源,正在被国内外果蝇研究领域的实验室所应用,对发育和疾病等相关研究发挥着广泛的促进作用。Drosophila melanogaster is an ideal model organism for diverse topics in biomedical research with various advantages. However,the limitation of genetic tools and fly strains always hampers further studies of related topics.To promote our research,we developed and optimized the CRISPR/Cas9 genome editing system,developed CRISPR/d Cas9 based transcriptional activation system and the next generation of transgenic RNAi technique in Drosophila. Equipped with these techniques,we can simply,efficiently and specifically regulate the genes of interest. Meanwhile,taking advantage of these novel genetic techniques,we built the library of mutant flies,transgenic transcriptional activation flies and transgenic RNAi flies. These genetic tools together with corresponding fly resources make Tsinghua Fly Center（ THFC） to be one of the most important centers of Drosophila techniques and resources around the world. Importantly,both these techniques and the fly strains are widely used in the entire Drosophila research field,and have broad impact on the research efforts in understanding development and diseases.

关 键 词：黑腹果蝇 遗传工具 果蝇资源 

分 类 号：Q95-33[生物学—动物学]