Lasiodiplodia theobromae激活芒果防御酶基因差异表达的研究  

Differential Expression of Mango Defense Enzyme Gene Activated by Lasiodiplodia theobromae

作　　者：康浩 苏初连[1,2] 梅志栋 杨石有 刘晓妹 蒲金基 张贺[2] KANG Hao;SU Chu-lian;MEI Zhi-dong;YANG Shi-you;LIU Xiao-mei;PU Jin-ji;ZHANG He(Institute of Tropical Agriculture and Forestry,Hainan University,Hainan Haikou 570228,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Integrated Pest Management on Tropical Grops,Ministry of Agriculture,Hainan Haikou 571101,China;Chengmai County Agriculture Bureau,Hainan Chengmai 571101,China)

机构地区：[1]海南大学热带农林学院,海南海口570228 [2]中国热带农业科学院环境与植物保护研究所,海南海口571101 [3]澄迈县农业局,海南澄迈571900

出　　处：《西南农业学报》2018年第7期1451-1455,共5页Southwest China Journal of Agricultural Sciences

基　　金：中央级公益性科研院所基本科研业务费专项(17CXTD-09;1630032016023;1630042017030;1630032017048);海南省重大科技计划项目(ZDKJ2017003)

摘　　要：【目的】为明确Lasiodiplodia theobromae侵染芒果组织的过程中对防御酶相关基因的诱导表达情况。【方法】本研究采用实时荧光定量PCR技术(qRT PCR)研究了Lasiodiplodia theobromae侵染芒果叶片和成熟果实时Mi POD、Mi PPO、Mi CHI、Mi PR1等7个防御酶基因表达情况。【结果】Lasiodiplodia theobromae侵染芒果果实后均不同程度的激活芒果抗病防御酶的活性,抗病防御酶相关基因的表达量极显著,均在被侵染36 h后达最大值,基因Mi POD表达量最高为0 h的33倍,Mi PAL为0 h的450倍;侵染叶片后,活性氧相关基因表达量极显著,均在被侵染24 h后达最大值,抗病相关基因中Mi POD和Mi PPO的表达量呈先上升后下降趋势,基因Mi CHI持续高表达,48 h时表达量达到最大值约为0 h的40倍,Mi PR1在接种72 h时出现最高表达量。【结论】研究表明,寄主植物感病后,Mi POD、Mi PPO、Mi CHI、Mi PR1等7个防御酶基因活性增强,增加寄主植物对病原菌的抵抗能力,进而延缓了病原菌在寄主组织内的迅速扩展。【Objective】The present study aimed to clarify the inducible expression of defense enzymes related genes in the process of infecting mango tissues with Lasiodiplodia theobromae.【Method】The expression changes of mango defense resistance enzyme gene was activated by Lasiodiplodia theobromae though quantitative real-time PCR.【Result】The disease resistant enzyme activities of mango were activated in different degrees after infected the fruit,and the expression of defense enzymes related genes was extremely significant and reached the maximum after being infected for 36 hours; Infected leaves,the maximum value of ROS related gene expression significantly infected after 24 hours reached,the expression of Mi POD and Mi PPO increased first and then decreased,and the expression of Mi CHI gene remained high,Mi PR1 showed the highest expression at 72 hours.【Conclusion】The host susceptible plants enhanced Mi POD,Mi PPO,Mi CHI,Mi PR1 and other 7 defense enzyme gene activity,increased the resistance of host plants to disease and delayed the rapid expansion of the pathogen in the host tissues.

关 键 词：芒果 LASIODIPLODIA theobromae 防御酶基因 

分 类 号：S667[农业科学—果树学]