雷公藤甲素超临界萃取工艺研究  

Study on supercritical extraction process of triptolide

作　　者：张璟 祝传书 张兴 ZHANG Jing;ZHU Chuan-shu;ZHANG Xing(Biorational Pesticide R and D Center,Northwest A and F University,Yangling 712100,China;Shaanxi Research Center of Biopesticide Technology,Yangling 712100,China)

机构地区：[1]西北农林科技大学无公害农药研究服务中心,陕西杨凌712100 [2]陕西省生物农药工程技术研究中心,陕西杨凌712100

出　　处：《河北农业大学学报》2018年第4期101-105,共5页Journal of Hebei Agricultural University

基　　金：国家自然科学基金(31272110)

摘　　要：为了建立快速提取检测雷公藤甲素的分析方法,本试验研究了雷公藤根皮、不定根和发状根中雷公藤甲素的超临界CO_2(SFE-CO_2)萃取工艺,并使用液相色谱质谱联用仪(HPLC-MS)检测雷公藤甲素含量。结果表明:超临界CO_2萃取根皮中甲素较适宜条件为:萃取压力48.265 MPa,萃取温度35℃,改性剂正丁醇、静态萃取时间15min及CO_2体积25 mL。超临界CO_2萃取不定根和发状根中甲素较适宜条件为:萃取压力27.580 MPa,萃取温度55℃,改性剂乙醇、静态萃取时间20 min及CO_2体积25 mL。添加回收率为80%~107%,相对标准偏差(RSD)为7.7%~10.6%。研究表明,超临界CO_2萃取全程分析时间小于1h,与常规溶剂法相比,该方法具有萃取效率高、无污染、经济等优点。In order to establish an analytical method for rapid extraction and detection of triptolide,the supercritical CO_2（SFE-CO_2）extraction process of triptolide in roots,adventitious roots and hairy roots of Tripterygium wilfordii were studied.The instrument（HPLC-MS）was used to detect the content of triptolide.The results showed that the optimum conditions for the extraction of triptolide in the root bark of supercritical CO_2 were as follows：extraction pressure was 48.265 MPa,extraction temperature was 35℃,modifier was n-butanol,static extraction time was 15 min and CO_2 volume was 25 mL.Supercritical CO_2 extraction of adventitious roots and hairy roots of triptolide suitable conditions were：extraction pressure was27.580 MPa,extraction temperature was 55 ℃,modifier was ethanol,static extraction time was 20 min and CO_2 volume was 25 mL.The recovery was 80% to 107%,and the relative standard deviation（RSD）was 7.7%to 10.6%.The research showed that the whole analysis time of supercritical CO_2 extraction was less than 1 h.Compared with the conventional solvent method,the method has the advantages of high extraction efficiency,no pollution and economy.

关 键 词：超临界萃取 雷公藤甲素 液质联用检测 

分 类 号：O652.63[理学—分析化学]