基于白藜芦醇还原特性的银基纳米载体制备及肿瘤细胞杀伤效应评价  

In-Situ Preparation of Resveratrol-Loaded Silver Nanoparticles and their Tumor Cytotoxicity

作　　者：成杰伟 黄赛朋[1] 温惠云[1] 许宁侠[1,2] 刘先宁 肖湘华 李文帅 薛伟明[1] CHENG Jie-wei;HUANG Sai-peng;WEN Hui-yun;XU Ning-xia;L;XIAO Xiang-hua;LI Wen-shuai;XUE Wei-ming(School of Chemical Engineering,Northwest University,Xi＇an 710069,China;Xi＇an International University,Xi＇an 710077,China;Shaa~xi Institute of Ophthalmology,Xi＇an 710002,China)

机构地区：[1]西北大学化工学院,陕西西安710069 [2]西安外事学院,陕西西安710077 [3]陕西省眼科研究所,陕西西安710002

出　　处：《高校化学工程学报》2018年第4期893-901,共9页Journal of Chemical Engineering of Chinese Universities

基　　金：陕西省教育厅专项科研计划项目(16JK1770);陕西省自然科学基金(2015JM2044;2014JQ2067);中国博士后科学基金第61批面上项目(2017M613190)

摘　　要：以反式白藜芦醇(resveratrol,RES)为还原剂,硝酸银(AgNO_3)为前驱体,十六烷基三甲基溴化铵(cetyltrimethylammonium bromide,CTAB)为表面活性剂和相转移催化剂,采用原位液相还原工艺制备了负载RES的银基纳米载体(RES-AgNPs)。当AgNO_3、RES和CTAB摩尔比为1:1:0.5、40℃反应13 h时,制得平均粒径(45.5±2)nm、zeta电位-21 mV的RES-AgNPs。红外光谱和紫外吸收光谱分析结果表明,RES成功负载在纳米银表面,负载量达到0.0883 mg?mg^(-1)。体外释放实验表明,RES-AgNPs具有过氧化氢(H_2O_2)敏感响应的释放特性,当p H=5.4且CH_2O_2=25μmol?L^(-1)时RES释放率为89%。以人乳腺癌细胞MCF-7为肿瘤细胞模型,MTT实验结果显示,当样品剂量均为15μg?mL^(-1)时,与RES、AgNPs和RES+AgNPs联合给药相比,RES-AgNPs对细胞的生长抑制率分别提高了62.6%、68.2%和55.1%,体现了纳米载体中银纳米粒子与负载药物RES对肿瘤细胞的协同杀伤效应。Silver nanoparticles（RES-AgNPs） were prepared by in-situ reduction using resveratrol（RES） as a reducing agent, silver nitrate as a precursor and cetyltrimethylammonium bromide（CTAB） as a dispersing agent and phase transfer catalyst. When studied with RES, AgNO3 and CTAB molar mass ratio of 1：1：0.5 at 40 for ℃13 h, the optimum RES-AgNPs prepared has an average size of 45.5 nm ± 2 nm and zeta value of-21 mV. Moreover, the infrared spectroscopy（IR） and ultraviolet visible（UV） spectrum results show that RES is successfully loaded on the surface of AgNPs with high loading efficiency of 0.0883 mg？mg^（-1）. Drug release measurement in vitro shows that RES-AgNPs is responsive to hydrogen peroxide H2 O2, and the release ratio is 89 % when hydrogen peroxide concentration is 25 μmol？L^（-1） at p H 5.4. Further evaluation of the therapeutic efficacy of RES-AgNPs was carried out in vitro by evaluating MCF-7 cell viability using MTT method. Compared with cell inhibition effects of RES, AgNPs and RES ＋ AgNPs, RES-AgNPs exhibits excellent synergistic antitumor effects with an improvement of 62.6 %, 68.2 % and 55.1 %, respectively.

关 键 词：白藜芦醇 纳米银 原位液相还原工艺 肿瘤细胞毒性 

分 类 号：TQ469[化学工程—制药化工] R944.9[医药卫生—药剂学]