新疆南疆牛乳头状瘤病毒1型SY-12株的基因鉴定及分析  

Gene Identification and Analysis of Bovine papillomavirus Type 1SY-12 Strain in South Xinjiang

作　　者：张婉琪[1] 张莹钰[1] 崔鑫[2] 朱广艺 宋健颖 胡建军[2] Zhang Wanqi;Zhang Yingyu;Cui Xin;Zhu Guangyi;Song Jianying;Hu Jianjun(College of Life Science,Tarim University,Alar,Xinjiang 843300;College of Animal Science,Tarim University,Alar,Xinjiang 843300)

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔8433003 [2]塔里木大学动物科学学院,新疆阿拉尔8433003

出　　处：《塔里木大学学报》2018年第2期1-6,共6页Journal of Tarim University

基　　金：国家自然科学基金项目(31660725;31160497);新疆生产建设兵团第一师科技局资助项目(2016XS03);新疆生产建设兵团科技局现代农业科技攻关与成果转化资助项目(2016AC012)

摘　　要：【目的】确定新疆南疆某养殖户疑似感染乳头状瘤病料基因型,探讨牛乳头状瘤(Bovine Papillomatosis,BP)的临床病理特征及鉴别诊断方法。【方法】采集新疆南疆某养殖户疑似感染乳头状瘤病料,进行病理组织学鉴定并进行基因鉴定分型。【结果】本实验采集病料经组织病理学和PCR鉴定分析,经测序确定该疑似感染病例为牛乳头状瘤病BPV-1型。【结论】对新疆南疆某养殖户患病牛进行检测,确定为牛乳头状瘤病毒1型感染,丰富该病流行病学资料为后续病毒全长基因序列测定及分析的研究奠定基础。【Objective】To determine the genotype of a suspected papilloma case from a farmer in southern Xinjiang,the clinicopathological characteristics and differential diagnosis of bovine papillomavirus（BP） were explored.【Method】The suspected papillomatosis samples from a farmer in southern Xinjiang were collected for histopathological identification and genotyping.【Result】The samples were identified by histopathology and PCR.The suspected case was confirmed to be BPV-1 type of bovine papillomatosis by sequencing.【Conclusion】The diseased cattle of a farmer in southern Xinjiang were detected and confirmed to be infected by bovine papillomavirus type 1.The epidemiological data of the disease were enriched to lay a foundation for the sequencing and analysis of the full-length gene of Bovine papillomavirus.

关 键 词：BPV 病理组织学 基因分型 

分 类 号：S852.65[农业科学—基础兽医学]